Identification of a Sterility-inducing Cytoplasm in a Fertile Accession Line of Phaseolus Vulgaris L

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1991 Feb 1

PMID 1706293

Citations 12

Authors

S A Mackenzie

Affiliations

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Abstract

Previous investigations into the genetic mechanism of fertility restoration in cytoplasmic male sterile Phaseolus vulgaris suggested that this is a particularly interesting system for the study of nuclear-mitochondrial interactions. This study was conducted to investigate the nature of nuclear-mitochondrial compatibility in fertile accession line G08063, the reported progenitor to the cytoplasmic male sterile line. Results from genetic analysis indicated that fertile line G08063 carried a sterility-inducing cytoplasm with a fertility restoring nuclear genotype. Mitochondrial DNA analysis indicated that the mechanism of fertility restoration by line G08063 was different from that conditioned by Fr, a previously described restorer gene. A mitochondrial DNA sequence associated with sterility and lost upon fertility restoration by nuclear gene Fr was present in the mitochondrial genome of fertile line G08063; this sequence was not carried within the mitochondrial genome of any other P. vulgaris accession line tested.

Citing Articles

A unique cytoplasmic male sterility (CMS) determinant is present in three Phaseolus species characterized by different mitochondrial genomes.

Hervieu F, Bannerot H, Pelletier G Theor Appl Genet. 2013; 88(3-4):314-20.

PMID: 24186012 DOI: 10.1007/BF00223638.

Counting mtDNA molecules in Phaseolus vulgaris: sublimons are constantly produced by recombination via short repeats and undergo rigorous selection during substoichiometric shifting.

Woloszynska M, Trojanowski D Plant Mol Biol. 2009; 70(5):511-21.

PMID: 19387845 DOI: 10.1007/s11103-009-9488-8.

A fragment of chloroplast DNA was transferred horizontally, probably from non-eudicots, to mitochondrial genome of Phaseolus.

Woloszynska M, Bocer T, Mackiewicz P, Janska H Plant Mol Biol. 2005; 56(5):811-20.

PMID: 15803417 DOI: 10.1007/s11103-004-5183-y.

Tracing evolutionary and developmental implications of mitochondrial stoichiometric shifting in the common bean.

Lyznik A, Woloszynska M, Janska H, Tohme J, Mackenzie S Genetics. 2001; 158(2):851-64.

PMID: 11404346 PMC: 1461663. DOI: 10.1093/genetics/158.2.851.

Construction and characterization of a common bean bacterial artificial chromosome library.

Vanhouten W, Mackenzie S Plant Mol Biol. 1999; 40(6):977-83.

PMID: 10527422 DOI: 10.1023/a:1006234823105.

References

Mackenzie S, Chase C . Fertility Restoration Is Associated with Loss of a Portion of the Mitochondrial Genome in Cytoplasmic Male-Sterile Common Bean. Plant Cell. 1990; 2(9):905-912. PMC: 159940. DOI: 10.1105/tpc.2.9.905. View