Emergence of a Bacterial Clone with Enhanced Virulence by Acquisition of a Phage Encoding a Secreted Phospholipase A2

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2006 Oct 18

PMID 17043230

Citations 32

Authors

Izabela Sitkiewicz

Michal J Nagiec

Paul Sumby

Stephanie D Butler

Colette Cywes-Bentley

James M Musser

Affiliations

Soon will be listed here.

Abstract

The molecular basis of pathogen clone emergence is relatively poorly understood. Acquisition of a bacteriophage encoding a previously unknown secreted phospholipase A(2) (designated SlaA) has been implicated in the rapid emergence in the mid-1980s of a new hypervirulent clone of serotype M3 group A Streptococcus. Although several lines of circumstantial evidence suggest that SlaA is a virulence factor, this issue has not been addressed experimentally. We found that an isogenic DeltaslaA mutant strain was significantly impaired in ability to adhere to and kill human epithelial cells compared with the wild-type parental strain. The mutant strain was less virulent for mice than the wild-type strain, and immunization with purified SlaA significantly protected mice from invasive disease. Importantly, the mutant strain was significantly attenuated for colonization in a monkey model of pharyngitis. We conclude that transductional acquisition of the ability of a GAS strain to produce SlaA enhanced the spread and virulence of the serotype M3 precursor strain. Hence, these studies identified a crucial molecular event underlying the evolution, rapid emergence, and widespread dissemination of unusually severe human infections caused by a distinct bacterial clone.

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