Genetically Encoded Ca2+ Indicators: Using Genetics and Molecular Design to Understand Complex Physiology

Overview

Journal J Physiol

Specialty Physiology

Date 2006 Oct 14

PMID 17038427

Citations 60

Authors

Michael I Kotlikoff

Affiliations

Soon will be listed here.

Abstract

This article reviews genetically encoded Ca2+ indicators (GECIs), with a focus on the use of these novel molecules in the context of understanding complex cell signalling in mammals, in vivo. The review focuses on the advantages and limitations of specific GECI design strategies and the results of experiments in which these molecules have been expressed in transgenic mice, concentrating particularly on recent experiments from our laboratory in which physiological signalling could be monitored in vivo. Finally, newer strategies for effective genetic specification of GECIs are briefly reviewed.

Citing Articles

Revisiting astrocytic calcium signaling in the brain.

Bai Y, Zhou Z, Han B, Xiang X, Huang W, Yao H Fundam Res. 2024; 4(6):1365-1374.

PMID: 39734522 PMC: 11670731. DOI: 10.1016/j.fmre.2023.11.021.

Calcium imaging: a technique to monitor calcium dynamics in biological systems.

Ghosh S, Dahiya M, Kumar A, Bheri M, Pandey G Physiol Mol Biol Plants. 2024; 29(12):1777-1811.

PMID: 38222278 PMC: 10784449. DOI: 10.1007/s12298-023-01405-6.

Intein-Mediated Protein Engineering for Cell-Based Biosensors.

Kang C, Shrestha K, Kwon S, Park S, Kim J, Kwon Y Biosensors (Basel). 2022; 12(5).

PMID: 35624584 PMC: 9138240. DOI: 10.3390/bios12050283.

Novel Optics-Based Approaches for Cardiac Electrophysiology: A Review.

Mullenbroich M, Kelly A, Acker C, Bub G, Bruegmann T, Bona A Front Physiol. 2021; 12:769586.

PMID: 34867476 PMC: 8637189. DOI: 10.3389/fphys.2021.769586.

Genetically engineered mice for combinatorial cardiovascular optobiology.

Lee F, Lee J, Shui B, Reining S, Jibilian M, Small D Elife. 2021; 10.

PMID: 34711305 PMC: 8555989. DOI: 10.7554/eLife.67858.

References

Copeland N, Jenkins N, Court D . Recombineering: a powerful new tool for mouse functional genomics. Nat Rev Genet. 2001; 2(10):769-79. DOI: 10.1038/35093556. View

Truong K, Sawano A, Mizuno H, Hama H, Tong K, Mal T . FRET-based in vivo Ca2+ imaging by a new calmodulin-GFP fusion molecule. Nat Struct Biol. 2001; 8(12):1069-73. DOI: 10.1038/nsb728. View

Heintz N . BAC to the future: the use of bac transgenic mice for neuroscience research. Nat Rev Neurosci. 2001; 2(12):861-70. DOI: 10.1038/35104049. View

Takahashi N, Nemoto T, Kimura R, Tachikawa A, Miwa A, Okado H . Two-photon excitation imaging of pancreatic islets with various fluorescent probes. Diabetes. 2002; 51 Suppl 1:S25-8. DOI: 10.2337/diabetes.51.2007.s25. View

Hancock J, Magee A, Childs J, Marshall C . All ras proteins are polyisoprenylated but only some are palmitoylated. Cell. 1989; 57(7):1167-77. DOI: 10.1016/0092-8674(89)90054-8. View

Eberhard M, Erne P . Kinetics of calcium binding to fluo-3 determined by stopped-flow fluorescence. Biochem Biophys Res Commun. 1989; 163(1):309-14. DOI: 10.1016/0006-291x(89)92136-0. View

Adams S, Harootunian A, Buechler Y, Taylor S, Tsien R . Fluorescence ratio imaging of cyclic AMP in single cells. Nature. 1991; 349(6311):694-7. DOI: 10.1038/349694a0. View

Eberhard M, Erne P . Calcium binding to fluorescent calcium indicators: calcium green, calcium orange and calcium crimson. Biochem Biophys Res Commun. 1991; 180(1):209-15. DOI: 10.1016/s0006-291x(05)81278-1. View

Ikura M, Clore G, Gronenborn A, Zhu G, Klee C, Bax A . Solution structure of a calmodulin-target peptide complex by multidimensional NMR. Science. 1992; 256(5057):632-8. DOI: 10.1126/science.1585175. View

10.

HEIM R, Tsien R . Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol. 1996; 6(2):178-82. DOI: 10.1016/s0960-9822(02)00450-5. View

11.

Siemering K, Golbik R, Sever R, Haseloff J . Mutations that suppress the thermosensitivity of green fluorescent protein. Curr Biol. 1996; 6(12):1653-63. DOI: 10.1016/s0960-9822(02)70789-6. View

12.

Romoser V, Hinkle P, Persechini A . Detection in living cells of Ca2+-dependent changes in the fluorescence emission of an indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence. A new class of fluorescent indicators. J Biol Chem. 1997; 272(20):13270-4. DOI: 10.1074/jbc.272.20.13270. View

13.

Dickson R, Cubitt A, Tsien R, Moerner W . On/off blinking and switching behaviour of single molecules of green fluorescent protein. Nature. 1997; 388(6640):355-8. DOI: 10.1038/41048. View

14.

Miyawaki A, Llopis J, HEIM R, McCaffery J, Adams J, Ikura M . Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature. 1997; 388(6645):882-7. DOI: 10.1038/42264. View

15.

Siegel M, Isacoff E . A genetically encoded optical probe of membrane voltage. Neuron. 1997; 19(4):735-41. DOI: 10.1016/s0896-6273(00)80955-1. View

16.

Miyawaki A, Griesbeck O, HEIM R, Tsien R . Dynamic and quantitative Ca2+ measurements using improved cameleons. Proc Natl Acad Sci U S A. 1999; 96(5):2135-40. PMC: 26749. DOI: 10.1073/pnas.96.5.2135. View

17.

Jurado L, Chockalingam P, Jarrett H . Apocalmodulin. Physiol Rev. 1999; 79(3):661-82. DOI: 10.1152/physrev.1999.79.3.661. View

18.

Baird G, Zacharias D, Tsien R . Circular permutation and receptor insertion within green fluorescent proteins. Proc Natl Acad Sci U S A. 1999; 96(20):11241-6. PMC: 18018. DOI: 10.1073/pnas.96.20.11241. View

19.

Shaner N, Campbell R, Steinbach P, Giepmans B, Palmer A, Tsien R . Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004; 22(12):1567-72. DOI: 10.1038/nbt1037. View

20.

Gross S, Piwnica-Worms D . Spying on cancer: molecular imaging in vivo with genetically encoded reporters. Cancer Cell. 2005; 7(1):5-15. DOI: 10.1016/j.ccr.2004.12.011. View