Swine Model of Carotid Artery Atherosclerosis: Experimental Induction by Surgical Partial Ligation and Dietary Hypercholesterolemia

Overview

Journal AJNR Am J Neuroradiol

Specialty Neurology

Date 2006 Oct 13

PMID 17032861

Citations 16

Authors

A Ishii

F Vinuela

Y Murayama

I Yuki

Y L Nien

D T Yeh

H V Vinters

Affiliations

Soon will be listed here.

Abstract

Introduction: Limited availability of a large animal model of carotid atherosclerosis has limited preclinical evaluation of endovascular therapeutic devices. The present study is aimed at developing such animal models with a novel approach, emphasizing the role of hemodynamics.

Methods: Using 18 carotid arteries from 9 miniswine, surgical partial ligation (approximately 80% stenosis) was carried out in untreated (group I; n = 6) and balloon-injured arteries (group II; n = 9). Three arteries were subjected to sham-operation for control (group III; n = 3). All animals were fed with a high-fat diet until sacrifice. Angiograms and histologic sections of the vessels were analyzed to evaluate both models.

Results: Atherosclerotic changes were confirmed in 6 of 6 in group I and 6 of 9 arteries in group II, whereas all in group III remained intact. Three arteries in group II resulted in thrombotic occlusion. Advanced plaques with intraplaque hemorrhage and/or calcification were seen in 4 of 6 arteries in group I but none in group II. The cross-sectional area stenosis and atherosclerotic stage for plaques in group I were both significantly higher than that in groups II and III.

Conclusion: In this series, surgical partial ligation with concomitant dietary hyperlipidemia is an appropriate experimental technique to develop advanced atherosclerotic plaques with minimal technical complications. This model showed no evidence of such benefits when applied in balloon-injured arteries.

Citing Articles

Development of a Model for Plaque Induction in Rat Carotid Arteries.

Wakako A, Sadato A, Oeda M, Higashiguchi S, Hayakawa M, Oshima M Asian J Neurosurg. 2023; 18(3):499-507.

PMID: 38152536 PMC: 10749859. DOI: 10.1055/s-0043-1763522.

Adaptive Wall Shear Stress Imaging in Phantoms, Simulations and In Vivo.

Karageorgos G, Kemper P, Lee C, Weber R, Kwon N, Meshram N IEEE Trans Biomed Eng. 2022; 70(1):154-165.

PMID: 35776824 PMC: 10103592. DOI: 10.1109/TBME.2022.3186854.

How to Establish a Minimal Invasive and Stable Carotid Artery Stenosis Rabbit Model? A Simple and Effective Carotid Artery Balloon Strain Technique.

Liu Z, Gong M, Zhao B, Gu J, Su H, Zhou Y Front Physiol. 2021; 12:752716.

PMID: 34803736 PMC: 8600145. DOI: 10.3389/fphys.2021.752716.

Feasibility of longitudinal monitoring of atherosclerosis with pulse wave imaging in a swine model.

Kemper P, Nauleau P, Karageorgos G, Weber R, Kwon N, Szabolcs M Physiol Meas. 2021; 42(10).

PMID: 34551396 PMC: 8733748. DOI: 10.1088/1361-6579/ac290f.

Computational Fluid Dynamics of Vascular Disease in Animal Models.

Acuna A, Berman A, Damen F, Meyers B, Adelsperger A, Bayer K J Biomech Eng. 2018; 140(8).

PMID: 29570754 PMC: 6993788. DOI: 10.1115/1.4039678.

References

Mas J, Chatellier G, Beyssen B . Carotid angioplasty and stenting with and without cerebral protection: clinical alert from the Endarterectomy Versus Angioplasty in Patients With Symptomatic Severe Carotid Stenosis (EVA-3S) trial. Stroke. 2003; 35(1):e18-20. DOI: 10.1161/01.STR.0000106913.33940.DD. View

Steele P, Chesebro J, W Stanson A, Holmes Jr D, Dewanjee M, Badimon L . Balloon angioplasty. Natural history of the pathophysiological response to injury in a pig model. Circ Res. 1985; 57(1):105-12. DOI: 10.1161/01.res.57.1.105. View

Shyy Y, Hsieh H, Usami S, Chien S . Fluid shear stress induces a biphasic response of human monocyte chemotactic protein 1 gene expression in vascular endothelium. Proc Natl Acad Sci U S A. 1994; 91(11):4678-82. PMC: 43851. DOI: 10.1073/pnas.91.11.4678. View

Narayanaswamy M, Wright K, Kandarpa K . Animal models for atherosclerosis, restenosis, and endovascular graft research. J Vasc Interv Radiol. 2000; 11(1):5-17. DOI: 10.1016/s1051-0443(07)61271-8. View

Roubin G, Yadav S, Iyer S, Vitek J . Carotid stent-supported angioplasty: a neurovascular intervention to prevent stroke. Am J Cardiol. 1996; 78(3A):8-12. DOI: 10.1016/s0002-9149(96)00487-0. View

Kraiss L, Geary R, Mattsson E, Vergel S, Au Y, Clowes A . Acute reductions in blood flow and shear stress induce platelet-derived growth factor-A expression in baboon prosthetic grafts. Circ Res. 1996; 79(1):45-53. DOI: 10.1161/01.res.79.1.45. View

Yamashita A, Furukoji E, Marutsuka K, Hatakeyama K, Yamamoto H, Tamura S . Increased vascular wall thrombogenicity combined with reduced blood flow promotes occlusive thrombus formation in rabbit femoral artery. Arterioscler Thromb Vasc Biol. 2004; 24(12):2420-4. DOI: 10.1161/01.ATV.0000147767.61336.de. View

REITMAN J, Mahley R . Changes induced in the lipoproteins of Yucatan miniature swine by cholesterol feeding. Biochim Biophys Acta. 1979; 575(3):446-57. DOI: 10.1016/0005-2760(79)90114-0. View

Hobson 2nd R . Update on the Carotid Revascularization Endarterectomy versus Stent Trial (CREST) protocol. J Am Coll Surg. 2002; 194(1 Suppl):S9-14. DOI: 10.1016/s1072-7515(01)01078-x. View

10.

Malek A, Jackman R, Rosenberg R, Izumo S . Endothelial expression of thrombomodulin is reversibly regulated by fluid shear stress. Circ Res. 1994; 74(5):852-60. DOI: 10.1161/01.res.74.5.852. View

11.

Malek A, Izumo S . Control of endothelial cell gene expression by flow. J Biomech. 1995; 28(12):1515-28. DOI: 10.1016/0021-9290(95)00099-2. View

12.

Passerini A, Polacek D, Shi C, Francesco N, Manduchi E, Grant G . Coexisting proinflammatory and antioxidative endothelial transcription profiles in a disturbed flow region of the adult porcine aorta. Proc Natl Acad Sci U S A. 2004; 101(8):2482-7. PMC: 356976. DOI: 10.1073/pnas.0305938101. View

13.

Gal D, Rongione A, Slovenkai G, Dejesus S, Lucas A, FIELDS C . Atherosclerotic Yucatan microswine: an animal model with high-grade, fibrocalcific, nonfatty lesions suitable for testing catheter-based interventions. Am Heart J. 1990; 119(2 Pt 1):291-300. DOI: 10.1016/s0002-8703(05)80019-5. View

14.

REITMAN J, Mahley R, FRY D . Yucatan miniature swine as a model for diet-induced atherosclerosis. Atherosclerosis. 1982; 43(1):119-32. DOI: 10.1016/0021-9150(82)90104-6. View

15.

Yadav J, Wholey M, Kuntz R, Fayad P, Katzen B, Mishkel G . Protected carotid-artery stenting versus endarterectomy in high-risk patients. N Engl J Med. 2004; 351(15):1493-501. DOI: 10.1056/NEJMoa040127. View

16.

Zarins C, Bomberger R, Glagov S . Local effects of stenoses: increased flow velocity inhibits atherogenesis. Circulation. 1981; 64(2 Pt 2):II221-7. View

17.

Schwartz R, Huber K, Murphy J, Edwards W, Camrud A, Vlietstra R . Restenosis and the proportional neointimal response to coronary artery injury: results in a porcine model. J Am Coll Cardiol. 1992; 19(2):267-74. DOI: 10.1016/0735-1097(92)90476-4. View

18.

Palmer R, Ashton D, Moncada S . Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature. 1988; 333(6174):664-6. DOI: 10.1038/333664a0. View

19.

Bonan R, Paiement P, Scortichini D, Cloutier M, Leung T . Coronary restenosis: evaluation of a restenosis injury index in a swine model. Am Heart J. 1993; 126(6):1334-40. DOI: 10.1016/0002-8703(93)90531-d. View

20.

Ku D, Giddens D, Zarins C, Glagov S . Pulsatile flow and atherosclerosis in the human carotid bifurcation. Positive correlation between plaque location and low oscillating shear stress. Arteriosclerosis. 1985; 5(3):293-302. DOI: 10.1161/01.atv.5.3.293. View