Time-dependent DNA Condensation Induced by Amyloid Beta-peptide

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2006 Oct 10

PMID 17028131

Citations 15

Authors

Haijia Yu

Jinsong Ren

Xiaogang Qu

Affiliations

Soon will be listed here.

Abstract

The major protein component of the amyloid deposition in Alzheimer's disease is a 39-43 residue peptide, amyloid beta (Abeta). Abeta is toxic to neurons, although the mechanism of neurodegeneration is uncertain. Evidence exists for non-B DNA conformation in the hippocampus of Alzheimer's disease brains, and Abeta was reportedly able to transform DNA conformation in vitro. In this study, we found that DNA conformation was altered in the presence of Abeta, and Abeta induced DNA condensation in a time-dependent manner. Furthermore, Abeta sheets, serving as condensation nuclei, were crucial for DNA condensation, and Cu(2+) and Zn(2+) ions inhibited Abeta sheet-induced DNA condensation. Our results suggest DNA condensation as a mechanism of Abeta toxicity.

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References

Krishnamoorthy G, Duportail G, Mely Y . Structure and dynamics of condensed DNA probed by 1,1'-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene)bis[4-[[3- methylbenz-1,3-oxazol-2-yl]methylidine]-1,4-dihydroquinolinium] tetraiodide fluorescence. Biochemistry. 2002; 41(51):15277-87. DOI: 10.1021/bi020440y. View

Xu Y, Szoka Jr F . Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection. Biochemistry. 1996; 35(18):5616-23. DOI: 10.1021/bi9602019. View

Arents G, Moudrianakis E . Topography of the histone octamer surface: repeating structural motifs utilized in the docking of nucleosomal DNA. Proc Natl Acad Sci U S A. 1993; 90(22):10489-93. PMC: 47802. DOI: 10.1073/pnas.90.22.10489. View

Bodles A, Guthrie D, Harriott P, Campbell P, Irvine G . Toxicity of non-abeta component of Alzheimer's disease amyloid, and N-terminal fragments thereof, correlates to formation of beta-sheet structure and fibrils. Eur J Biochem. 2000; 267(8):2186-94. DOI: 10.1046/j.1432-1327.2000.01219.x. View

Golabek A, Soto C, Vogel T, Wisniewski T . The interaction between apolipoprotein E and Alzheimer's amyloid beta-peptide is dependent on beta-peptide conformation. J Biol Chem. 1996; 271(18):10602-6. DOI: 10.1074/jbc.271.18.10602. View

Zeng H, Chen Q, Zhao B . Genistein ameliorates beta-amyloid peptide (25-35)-induced hippocampal neuronal apoptosis. Free Radic Biol Med. 2004; 36(2):180-8. DOI: 10.1016/j.freeradbiomed.2003.10.018. View

CRAPPER D, Quittkat S, De Boni U . Altered chromatin conformation in Alzheimer's disease. Brain. 1979; 102(3):483-95. DOI: 10.1093/brain/102.3.483. View

Hegde M, Anitha S, Latha K, Mustak M, Stein R, Ravid R . First evidence for helical transitions in supercoiled DNA by amyloid Beta Peptide (1-42) and aluminum: a new insight in understanding Alzheimer's disease. J Mol Neurosci. 2004; 22(1-2):19-31. DOI: 10.1385/jmn:22:1-2:19. View

Li X, Peng Y, Qu X . Carbon nanotubes selective destabilization of duplex and triplex DNA and inducing B-A transition in solution. Nucleic Acids Res. 2006; 34(13):3670-6. PMC: 1540735. DOI: 10.1093/nar/gkl513. View

10.

Nandi P, Sizaret P . Murine recombinant prion protein induces ordered aggregation of linear nucleic acids to condensed globular structures. Arch Virol. 2001; 146(2):327-45. DOI: 10.1007/s007050170178. View

11.

Zou J, Kajita K, Sugimoto N . Cu Inhibits the Aggregation of Amyloid β-Peptide(1-42) in vitro. Angew Chem Int Ed Engl. 2018; 40(12):2274-2277. DOI: 10.1002/1521-3773(20010618)40:12<2274::AID-ANIE2274>3.0.CO;2-5. View

12.

Lambert M, Barlow A, Chromy B, Edwards C, Freed R, Liosatos M . Diffusible, nonfibrillar ligands derived from Abeta1-42 are potent central nervous system neurotoxins. Proc Natl Acad Sci U S A. 1998; 95(11):6448-53. PMC: 27787. DOI: 10.1073/pnas.95.11.6448. View

13.

McKenzie D, Kwok K, Rice K . A potent new class of reductively activated peptide gene delivery agents. J Biol Chem. 2000; 275(14):9970-7. DOI: 10.1074/jbc.275.14.9970. View

14.

Blanc E, Toborek M, Mark R, Hennig B, Mattson M . Amyloid beta-peptide induces cell monolayer albumin permeability, impairs glucose transport, and induces apoptosis in vascular endothelial cells. J Neurochem. 1997; 68(5):1870-81. DOI: 10.1046/j.1471-4159.1997.68051870.x. View

15.

Nandi P, Leclerc E . Polymerization of murine recombinant prion protein in nucleic acid solution. Arch Virol. 1999; 144(9):1751-63. DOI: 10.1007/s007050050702. View

16.

Kim H, Chae S, Chromy B, Lee S, Park Y, Klein W . Selective neuronal degeneration induced by soluble oligomeric amyloid beta protein. FASEB J. 2002; 17(1):118-20. DOI: 10.1096/fj.01-0987fje. View

17.

Church G, Sussman J, Kim S . Secondary structural complementarity between DNA and proteins. Proc Natl Acad Sci U S A. 1977; 74(4):1458-62. PMC: 430798. DOI: 10.1073/pnas.74.4.1458. View

18.

Thual C, Komar A, Bousset L, Cullin C, Melki R . Structural characterization of Saccharomyces cerevisiae prion-like protein Ure2. J Biol Chem. 1999; 274(19):13666-74. DOI: 10.1074/jbc.274.19.13666. View

19.

Pike C, BURDICK D, Walencewicz A, Glabe C, Cotman C . Neurodegeneration induced by beta-amyloid peptides in vitro: the role of peptide assembly state. J Neurosci. 1993; 13(4):1676-87. PMC: 6576726. View

20.

Masters C, Simms G, Weinman N, Multhaup G, McDonald B, Beyreuther K . Amyloid plaque core protein in Alzheimer disease and Down syndrome. Proc Natl Acad Sci U S A. 1985; 82(12):4245-9. PMC: 397973. DOI: 10.1073/pnas.82.12.4245. View