Injection of the Sciatic Nerve with TMEV: a New Model for Peripheral Nerve Demyelination

Overview

Journal Virology

Specialty Microbiology

Date 2006 Oct 10

PMID 17028060

Citations 6

Authors

Kristen M Drescher

Steven M Tracy

Affiliations

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Abstract

Demyelination of the human peripheral nervous system (PNS) can be caused by diverse mechanisms including viral infection. Despite association of several viruses with the development of peripheral demyelination, animal models of the condition have been limited to disease that is either autoimmune or genetic in origin. We describe here a model of PNS demyelination based on direct injection of sciatic nerves of mice with the cardiovirus, Theiler's murine encephalomyelitis virus (TMEV). Sciatic nerves of FVB mice develop inflammatory cell infiltration following TMEV injection. Schwann cells and macrophages are infected with TMEV. Viral replication is observed initially in the sciatic nerves and subsequently the spinal cord. Sciatic nerves are demyelinated by day 5 post-inoculation (p.i.). Injecting sciatic nerves of scid mice resulted in increased levels of virus recovered from the sciatic nerve and spinal cord relative to FVB mice. Demyelination also occurred in scid mice and by 12 days p.i., hindlimbs were paralyzed. This new model of virus-induced peripheral demyelination may be used to dissect processes involved in protection of the PNS from viral insult and to study the early phases of lesion development.

Citing Articles

Surgically Induced Demyelination in Rat Sciatic Nerve.

Rayilla R, Naidu M, Prakash Babu P Brain Sci. 2023; 13(5).

PMID: 37239226 PMC: 10216806. DOI: 10.3390/brainsci13050754.

Host genetic diversity drives variable central nervous system lesion distribution in chronic phase of Theiler's Murine Encephalomyelitis Virus (TMEV) infection.

Lawley K, Rech R, Elenwa F, Han G, Perez Gomez A, Amstalden K PLoS One. 2021; 16(8):e0256370.

PMID: 34415947 PMC: 8378701. DOI: 10.1371/journal.pone.0256370.

Comparison of Theiler's Murine Encephalomyelitis Virus Induced Spinal Cord and Peripheral Nerve Lesions Following Intracerebral and Intraspinal Infection.

Jin W, Leitzen E, Goebbels S, Nave K, Baumgartner W, Hansmann F Int J Mol Sci. 2019; 20(20).

PMID: 31623261 PMC: 6834305. DOI: 10.3390/ijms20205134.

Virus-triggered spinal cord demyelination is followed by a peripheral neuropathy resembling features of Guillain-Barré Syndrome.

Leitzen E, Raddatz B, Jin W, Goebbels S, Nave K, Baumgartner W Sci Rep. 2019; 9(1):4588.

PMID: 30872675 PMC: 6418285. DOI: 10.1038/s41598-019-40964-1.

The L-coding region of the DA strain of Theiler's murine encephalomyelitis virus causes dysfunction and death of myelin-synthesizing cells.

Ghadge G, Wollmann R, Baida G, Traka M, Roos R J Virol. 2011; 85(18):9377-84.

PMID: 21752920 PMC: 3165738. DOI: 10.1128/JVI.00178-11.

References

Rodriguez M, Nabozny G, Thiemann R, David C . Influence of deletion of T cell receptor V beta genes on the Theiler's virus model of multiple sclerosis. Autoimmunity. 1994; 19(4):221-30. DOI: 10.3109/08916939409071347. View

Yaghootfam A, Gieselmann V, Eckhardt M . Delay of myelin formation in arylsulphatase A-deficient mice. Eur J Neurosci. 2005; 21(3):711-20. DOI: 10.1111/j.1460-9568.2005.03891.x. View

Tsunoda I, Wada Y, Libbey J, Cannon T, Whitby F, Fujinami R . Prolonged gray matter disease without demyelination caused by Theiler's murine encephalomyelitis virus with a mutation in VP2 puff B. J Virol. 2001; 75(16):7494-505. PMC: 114985. DOI: 10.1128/JVI.75.16.7494-7505.2001. View

Rodriguez M, Dunkel A, Thiemann R, Leibowitz J, Zijlstra M, Jaenisch R . Abrogation of resistance to Theiler's virus-induced demyelination in H-2b mice deficient in beta 2-microglobulin. J Immunol. 1993; 151(1):266-76. View

Zhu Y, Ljunggren H, Mix E, Li H, van der Meide P, Elhassan A . Suppression of autoimmune neuritis in IFN-gamma receptor-deficient mice. Exp Neurol. 2001; 169(2):472-8. DOI: 10.1006/exnr.2001.7662. View

Theiler M, Gard S . ENCEPHALOMYELITIS OF MICE : III. EPIDEMIOLOGY. J Exp Med. 2009; 72(1):79-90. PMC: 2135011. DOI: 10.1084/jem.72.1.79. View

Lindsley M, Thiemann R, Rodriguez M . Enumeration and distribution of T-cell subsets, macrophages, and IgG positive cells in the CNS of SJL/J mice infected with Theiler's virus. Ann N Y Acad Sci. 1988; 540:657-60. DOI: 10.1111/j.1749-6632.1988.tb27203.x. View

Song G, Jia W . The heterogeneity in the immune response and efficiency of viral dissemination in brain infected with herpes simplex virus type 1 through peripheral or central route. Acta Neuropathol. 1999; 97(6):649-56. DOI: 10.1007/s004010051042. View

Theiler M . SPONTANEOUS ENCEPHALOMYELITIS OF MICE, A NEW VIRUS DISEASE. J Exp Med. 2009; 65(5):705-19. PMC: 2133518. DOI: 10.1084/jem.65.5.705. View

10.

Solomon T, Willison H . Infectious causes of acute flaccid paralysis. Curr Opin Infect Dis. 2003; 16(5):375-81. DOI: 10.1097/00001432-200310000-00002. View

11.

Lindsley M, Thiemann R, Rodriguez M . Cytotoxic T cells isolated from the central nervous systems of mice infected with Theiler's virus. J Virol. 1991; 65(12):6612-20. PMC: 250726. DOI: 10.1128/JVI.65.12.6612-6620.1991. View

12.

Sabin A . Pathogenesis of poliomyelitis; reappraisal in the light of new data. Science. 1956; 123(3209):1151-7. DOI: 10.1126/science.123.3209.1151. View

13.

Patick A, Pease L, David C, Rodriguez M . Major histocompatibility complex-conferred resistance to Theiler's virus-induced demyelinating disease is inherited as a dominant trait in B10 congenic mice. J Virol. 1990; 64(11):5570-6. PMC: 248609. DOI: 10.1128/JVI.64.11.5570-5576.1990. View

14.

Jin L, Perng G, Brick D, Naito J, Nesburn A, Jones C . Methods for detecting the HSV-1 LAT anti-apoptosis activity in virus infected tissue culture cells. J Virol Methods. 2004; 118(1):9-13. DOI: 10.1016/j.jviromet.2004.01.011. View

15.

Martinat C, Jarousse N, Prevost M, Brahic M . The GDVII strain of Theiler's virus spreads via axonal transport. J Virol. 1999; 73(7):6093-8. PMC: 112673. DOI: 10.1128/JVI.73.7.6093-6098.1999. View

16.

Rodriguez M, Pavelko K, Coffman R . Gamma interferon is critical for resistance to Theiler's virus-induced demyelination. J Virol. 1995; 69(11):7286-90. PMC: 189655. DOI: 10.1128/JVI.69.11.7286-7290.1995. View

17.

Tanimura N, Imada T, Kashiwazaki Y, Shahirudin S, Sharifah S, Aziz A . Monoclonal antibody-based immunohistochemical diagnosis of Malaysian Nipah virus infection in pigs. J Comp Pathol. 2004; 131(2-3):199-206. DOI: 10.1016/j.jcpa.2004.03.006. View

18.

Drescher K, Kono K, Bopegamage S, Carson S, Tracy S . Coxsackievirus B3 infection and type 1 diabetes development in NOD mice: insulitis determines susceptibility of pancreatic islets to virus infection. Virology. 2004; 329(2):381-94. DOI: 10.1016/j.virol.2004.06.049. View

19.

Rao V, Thomas F . Neurological complications of HIV/AIDS. BETA. 2005; 17(2):37-46. View

20.

Rodriguez M, David C . Demyelination induced by Theiler's virus: influence of the H-2 haplotype. J Immunol. 1985; 135(3):2145-8. View