Neuronal Acetylcholine Receptor Channels from Insects: a Comparative Electrophysiological Study

Overview

Journal J Comp Physiol A

Specialties Neurology
Physiology
Social Sciences

Date 1990 Sep 1

PMID 1701832

Citations 4

Authors

E Tareilus

W Hanke

H Breer

Affiliations

Soon will be listed here.

Abstract

The channel properties of nicotinic acetylcholine receptor subtypes in the nervous system of insects (Locusta migratoria) have been characterized. Single channel measurements were performed using patch-clamp techniques as well as planar lipid bilayer reconstitution approaches. In reconstitution experiments using receptor-preparations isolated from neuronal membranes by alpha-toxin affinity chromatography, a ligand-gated channel type was found, which showed a high conductance and a short mean lifetime. Patch-clamp experiments on synapse-free somata of isolated nerve cells revealed an acetylcholine-gated channel type with a lower conductance but a longer lifetime. The two different agonist-activated channel types are supposed to represent synaptic and extrasynaptic acetylcholine receptors.

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