[Analysis of Volatile Constituents from Leaves of Plants by Gas Chromatography/mass Spectrometry with Solid-phase Microextraction]

A method for analyzing volatile constituents from plant leaves with gas chromatography/mass spectrometry (GC/MS) coupled with solid-phase microextraction (SPME) was developed. The volatile compounds from the plant leaves inside a sealed flask maintained at 45 degrees C in a water bath were efficiently extracted with Polyacrylate (85 microm) SPME fibers prior to perform GC/MS analysis. The GC/MS analysis indicated that the volatile compounds from the plant leaves which is easy to be damaged by Tetraychus vienneis include relative large amounts of cis-3-hexenyl ester acetic acid, cis-3-hexenyl ester butanoic acid and alpha-famesene. These compounds were preliminarily confirmed to be accountable for attracting Tetraychus vienneis. This finding may lead to identify biological species for preventing and treating Tetraychus vienneis.