Immunohistological Identification of Interleukin-1 Activated Chondrocytes

Overview

Journal Ann Rheum Dis

Specialty Rheumatology

Date 1990 Nov 1

PMID 1701622

Citations 6

Authors

J T DINGLE

M E Davies

B Y Mativi

H F Middleton

Affiliations

Soon will be listed here.

Abstract

Interleukin-1 activated chondrocytes have been identified in pig articular cartilage by immunolocalisation using a polyclonal antiserum recognising cytokine induced surface epitopes. Although chondrocytes with membrane staining were noted in all zones of the cartilage, only a proportion of the cells were positive within the range of antiserum dilution used (1:100-1:2000). Maximum staining was found after four days' treatment of cultured cartilage with pig IL-1 alpha, though weak staining was just detectable after treatment for one day at high antiserum concentration. Immunoreactivity was also shown in cartilage which had been cultured with human recombinant IL-1 alpha.

Citing Articles

ICAM-1 expression on chondrocytes in rheumatoid arthritis: induction by synovial cytokines.

Davies M, Sharma H, Pigott R Mediators Inflamm. 1992; 1(1):71-4.

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Interleukin 1-induced calcium signalling in chondrocytes requires focal adhesions.

Luo L, Cruz T, McCulloch C Biochem J. 1997; 324 ( Pt 2):653-8.

PMID: 9182730 PMC: 1218478. DOI: 10.1042/bj3240653.

Expression of ICAM-1 on intact cartilage and isolated chondrocytes.

Bujia J, Behrends U, Rotter N, Pitzke P, Wilmes E, Hammer C In Vitro Cell Dev Biol Anim. 1996; 32(2):116-22.

PMID: 8907125 DOI: 10.1007/BF02723043.

Chondrocyte-peripheral blood mononuclear cell interactions: the role of ICAM-1.

Horner A, Davies M, Franz B Immunology. 1995; 86(4):584-90.

PMID: 8567025 PMC: 1384059.

Metalloproteinase production by rabbit articular cartilage: comparison of the effects of interleukin-1 alpha in vitro and in vivo.

Hembry R, Bagga M, DINGLE J, Thomas P, Reynolds J Virchows Arch. 1994; 425(4):413-24.

PMID: 7529620 DOI: 10.1007/BF00189580.

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