Application of Mutated Clostridium Difficile Toxin A for Determination of Glucosyltransferase-dependent Effects

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2006 Sep 22

PMID 16988280

Citations 22

Authors

Matthias Teichert

Helma Tatge

Janett Schoentaube

Ingo Just

Ralf Gerhard

Affiliations

Soon will be listed here.

Abstract

Mutation of tryptophan-101 in Clostridium difficile toxin A, a 308-kDa glucosyltransferase, resulted in a 50-fold-reduced cytopathic activity in cell culture experiments. The mutant toxin A was characterized and applied to distinguish between glucosyltransferase-dependent and -independent effects with respect to RhoB up-regulation as a cellular stress response.

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