Expression of Transforming Growth Factor-alpha in Primary Human Colon and Lung Carcinomas

Overview

Journal Br J Cancer

Specialty Oncology

Date 1990 Sep 1

PMID 1698444

Citations 13

Authors

C Liu

A Woo

M S Tsao

Affiliations

Soon will be listed here.

Abstract

The expression of TGF-alpha in human colon and lung carcinoma cell lines has been reported previously, but its expression in primary tumours has not been described in detail. We have used the radio-immunoassay method to measure the specific content of immunoreactive TGF-alpha in the acid ethanol extracts of normal and cancerous tissues of human colon and lung. The average TGF-alpha content of colon carcinomas is 4 times that of the normal mucosa, and for non-small cell lung carcinomas it is twice that of the normal parenchyma. Because of variability in the TGF-alpha expression among individuals and in different segments of colon and lobes of lung, the ratio of TGF-alpha content of paired tumour and normal tissue was also calculated. On average, the tumour/normal ratio for colon carcinoma is higher than that for lung carcinoma. Although 55% of colon tumours show a ratio 4 times, or greater, only 33% of lung carcinomas demonstrate this ratio. The level of TGF-alpha in both colon and lung carcinomas does not correlate with histological type stage, grade nor degree of desmoplasia of these tumours. Northern blot analysis of total cellular RNA confirms the expression of an approximately 4.8 kb TGF-alpha mRNA in normal colonic mucosa and lung parenchyma. However, in contrast to the results of radio-immunoassay, significant over-expression of TGF-alpha mRNA is uncommon in primary human colon carcinomas.

Citing Articles

Effects of calcium and vitamin D3 on transforming growth factors in rectal mucosa of sporadic colorectal adenoma patients: a randomized controlled trial.

Tu H, Flanders W, Ahearn T, Daniel C, Gonzalez-Feliciano A, Long Q Mol Carcinog. 2013; 54(4):270-80.

PMID: 24166893 PMC: 4484733. DOI: 10.1002/mc.22096.

TGF-alpha as a candidate tumor antigen for renal cell carcinomas.

Pelletier S, Tanguay S, Lee S, Gunaratnam L, Arbour N, Lapointe R Cancer Immunol Immunother. 2008; 58(8):1207-18.

PMID: 19052740 PMC: 11031064. DOI: 10.1007/s00262-008-0630-2.

Transgenic mice reveal roles for TGFalpha and EGF receptor in mammary gland development and neoplasia.

Schroeder J, Lee D J Mammary Gland Biol Neoplasia. 1997; 2(2):119-29.

PMID: 10882298 DOI: 10.1023/a:1026347629876.

Immunolocalization of transforming growth factor alpha in normal human tissues.

Christensen M, Poulsen S Histochem Cell Biol. 1996; 105(5):391-400.

PMID: 8781993 DOI: 10.1007/BF01463660.

Proto-oncogene and growth factor/receptor expression in the establishment of primary human non-small cell lung carcinoma cell lines.

Liu C, Tsao M Am J Pathol. 1993; 142(2):413-23.

PMID: 8382008 PMC: 1886714.

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