Translation of LINE-1 DNA Elements in Vitro and in Human Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Sep 1

PMID 1698287

Citations 66

Authors

D M Leibold

G D Swergold

M F Singer

R E Thayer

B A Dombroski

T G Fanning

Affiliations

Soon will be listed here.

Abstract

The LINE-1 (L1) family of interspersed DNA sequences found throughout the human genome (L1 Homo sapiens, L1Hs) includes active transposable elements. Current models for the mechanism of transposition involve reverse transcription of an RNA intermediate and utilization of element-encoded proteins. We report that an antiserum against the polypeptide encoded by the L1Hs 5' open reading frame (ORF1) detects, in human cells, an endogenous ORF1 protein as well as the ORF1 product of an appropriate transfecting recombinant vector. The endogenous polypeptide is most abundant in teratocarcinoma and choriocarcinoma cells, among those cell lines tested; it appears to be a single species of approximately 38 kDa. In contrast, RNAs synthesized in vitro from cDNAs representing full-length, polyadenylylated cytoplasmic L1Hs RNA yield, upon in vitro translation, ORF1 products of slightly different sizes. This is consistent with the fact that the various cDNAs are different and represent transcription of different genomic L1Hs elements. In vitro studies additionally suggest that translation of ORF1 is initiated at the first AUG codon. Finally, in no case was an ORF1-ORF2 fusion protein detected.

Citing Articles

Advances in transposable elements: from mechanisms to applications in mammalian genomics.

Han M, Perkins M, Novaes L, Xu T, Chang H Front Genet. 2023; 14:1290146.

PMID: 38098473 PMC: 10719622. DOI: 10.3389/fgene.2023.1290146.

A novel role of TRIM28 B box domain in L1 retrotransposition and ORF2p-mediated cDNA synthesis.

Du Q, Stow E, LaCoste D, Freeman B, Baddoo M, Shareef A Nucleic Acids Res. 2023; 51(9):4429-4450.

PMID: 37070200 PMC: 10201437. DOI: 10.1093/nar/gkad247.

Affinity-Based Interactome Analysis of Endogenous LINE-1 Macromolecules.

Di Stefano L, Saba L, Oghbaie M, Jiang H, McKerrow W, Benitez-Guijarro M Methods Mol Biol. 2022; 2607:215-256.

PMID: 36449166 DOI: 10.1007/978-1-0716-2883-6_12.

Analysis of epigenetic features characteristic of L1 loci expressed in human cells.

Freeman B, White T, Kaul T, Stow E, Baddoo M, Ungerleider N Nucleic Acids Res. 2022; 50(4):1888-1907.

PMID: 35100410 PMC: 8887483. DOI: 10.1093/nar/gkac013.

Human transposon insertion profiling by sequencing (TIPseq) to map LINE-1 insertions in single cells.

McKerrow W, Tang Z, Steranka J, Payer L, Boeke J, Keefe D Philos Trans R Soc Lond B Biol Sci. 2020; 375(1795):20190335.

PMID: 32075555 PMC: 7061987. DOI: 10.1098/rstb.2019.0335.

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