Synthesis and Characterization of Cholesterol-poly(ethylene Glycol)-poly(D,L-lactic Acid) Copolymers for Promoting Osteoblast Attachment and Proliferation

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2006 Sep 16

PMID 16977387

Citations 1

Authors

Guanhua Yu

Jian Ji

Jiacong Shen

Affiliations

Soon will be listed here.

Abstract

A novel cholesterol-poly(ethylene glycol)-poly(D,L-lactic acid) copolymer (CPEG-PLA) has been synthesized as a potential surface additive for promoting osteoblast attachment and proliferation. The gel permeation chromatography (GPC) and nuclear magnetic resonance spectroscopy (NMR) results indicated the product had expected structure with low polydispersities in the range of 1.1-1.5. By blending the poly(D,L-lactic acid) (PLA) with CPEG-PLA, the surface of modified PLA membrane was investigated by atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle. The results revealed the enrichment of PEG chain on the surface. Osteoblast cell line (MC3T3) was chosen to test the cell behavior on modified PLA membranes. The osteoblast test about cell attachment, proliferation, cell viability and cell morphology investigation on CPEG-PLA modified PLA substrates showed the CPEG-PLA with 15 and 5 ethylene glycol units promoted osteoblast attachment and growth, while the CPEG-PLA with 30 ethylene glycol units prevent osteoblast adhesion and proliferation. This simple surface treatment method may have potentials for tissue engineering and other biomedical applications.

Citing Articles

Effect of PEG grafting density on surface properties of polyurethane substrata and the viability of osteoblast and fibroblast cells.

Abreu-Rejon A, Herrera-Kao W, May-Pat A, Avila-Ortega A, Rodriguez-Fuentes N, Uribe-Calderon J J Mater Sci Mater Med. 2022; 33(6):45.

PMID: 35585216 PMC: 9117377. DOI: 10.1007/s10856-022-06668-1.

References

Blum R, Wright R . Detection of pyuria and bacteriuria in symptomatic ambulatory women. J Gen Intern Med. 1992; 7(2):140-4. DOI: 10.1007/BF02598002. View

Salgado A, Coutinho O, Reis R . Bone tissue engineering: state of the art and future trends. Macromol Biosci. 2004; 4(8):743-65. DOI: 10.1002/mabi.200400026. View

Gopalakrishna P, Chaubey S, Manogaran P, Pande G . Modulation of alpha5beta1 integrin functions by the phospholipid and cholesterol contents of cell membranes. J Cell Biochem. 2000; 77(4):517-28. View

Zhu H, Ji J, Tan Q, Barbosa M, Shen J . Surface engineering of poly(DL-lactide) via electrostatic self-assembly of extracellular matrix-like molecules. Biomacromolecules. 2003; 4(2):378-86. DOI: 10.1021/bm025773p. View

Bancel S, Hu W . Confocal laser scanning microscopy examination of cell distribution in macroporous microcarriers. Biotechnol Prog. 1996; 12(3):398-402. DOI: 10.1021/bp960023a. View

Borden M, Attawia M, Khan Y, Laurencin C . Tissue engineered microsphere-based matrices for bone repair: design and evaluation. Biomaterials. 2002; 23(2):551-9. DOI: 10.1016/s0142-9612(01)00137-5. View

Lieb E, Hacker M, Tessmar J, Kunz-Schughart L, Fiedler J, Dahmen C . Mediating specific cell adhesion to low-adhesive diblock copolymers by instant modification with cyclic RGD peptides. Biomaterials. 2004; 26(15):2333-41. DOI: 10.1016/j.biomaterials.2004.07.010. View

Cook A, Hrkach J, Gao N, Johnson I, Pajvani U, Cannizzaro S . Characterization and development of RGD-peptide-modified poly(lactic acid-co-lysine) as an interactive, resorbable biomaterial. J Biomed Mater Res. 1997; 35(4):513-23. DOI: 10.1002/(sici)1097-4636(19970615)35:4<513::aid-jbm11>3.0.co;2-c. View

Tessmar J, Mikos A, Gopferich A . Amine-reactive biodegradable diblock copolymers. Biomacromolecules. 2002; 3(1):194-200. DOI: 10.1021/bm015608u. View

10.

Otsuka H, Nagasaki Y, Kataoka K . Characterization of aldehyde-PEG tethered surfaces: influence of PEG chain length on the specific biorecognition. Langmuir. 2004; 20(26):11285-7. DOI: 10.1021/la0483414. View

11.

Zhu H, Ji J, Lin R, Gao C, Feng L, Shen J . Surface engineering of poly(DL-lactic acid) by entrapment of alginate-amino acid derivatives for promotion of chondrogenesis. Biomaterials. 2002; 23(15):3141-8. DOI: 10.1016/s0142-9612(02)00058-3. View

12.

Simons K, Ikonen E . How cells handle cholesterol. Science. 2000; 290(5497):1721-6. DOI: 10.1126/science.290.5497.1721. View

13.

Risbud M, Bhonde M, Bhonde R . Effect of chitosan-polyvinyl pyrrolidone hydrogel on proliferation and cytokine expression of endothelial cells: implications in islet immunoisolation. J Biomed Mater Res. 2001; 57(2):300-5. DOI: 10.1002/1097-4636(200111)57:2<300::aid-jbm1171>3.0.co;2-q. View

14.

Tessmar J, Mikos A, Gopferich A . The use of poly(ethylene glycol)-block-poly(lactic acid) derived copolymers for the rapid creation of biomimetic surfaces. Biomaterials. 2003; 24(24):4475-86. DOI: 10.1016/s0142-9612(03)00345-4. View

15.

Chen C, Mrksich M, Huang S, Whitesides G, Ingber D . Geometric control of cell life and death. Science. 1997; 276(5317):1425-8. DOI: 10.1126/science.276.5317.1425. View

16.

Freed L, Marquis J, Nohria A, Emmanual J, Mikos A, Langer R . Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. J Biomed Mater Res. 1993; 27(1):11-23. DOI: 10.1002/jbm.820270104. View

17.

Wan Y, Chen W, Yang J, Bei J, Wang S . Biodegradable poly(L-lactide)-poly(ethylene glycol) multiblock copolymer: synthesis and evaluation of cell affinity. Biomaterials. 2003; 24(13):2195-203. DOI: 10.1016/s0142-9612(03)00107-8. View

18.

Hwang J, Iyer S, Li L, Claussen R, Harrington D, Stupp S . Self-assembling biomaterials: liquid crystal phases of cholesteryl oligo(L-lactic acid) and their interactions with cells. Proc Natl Acad Sci U S A. 2002; 99(15):9662-7. PMC: 124968. DOI: 10.1073/pnas.152667399. View

19.

Wang D, Ji J, Gao C, Yu G, Feng L . Surface coating of stearyl poly(ethylene oxide) coupling-polymer on polyurethane guiding catheters with poly(ether urethane) film-building additive for biomedical applications. Biomaterials. 2001; 22(12):1549-62. DOI: 10.1016/s0142-9612(00)00311-2. View

20.

Dalsin J, Hu B, Lee B, Messersmith P . Mussel adhesive protein mimetic polymers for the preparation of nonfouling surfaces. J Am Chem Soc. 2003; 125(14):4253-8. DOI: 10.1021/ja0284963. View