Effect of Repeated Freeze-thaw Cycles on Routine Plasma Biochemical Constituents in Canine Plasma

Background: The effect of repeated freeze-thaw cycles on plasma constituents has not been assessed in dogs, although such a procedure is not uncommon to use in routine laboratory practice.

Objective: To assess the effect of freeze-thaw cycles on routine plasma constituents in healthy dogs.

Methods: Six healthy adult dogs were used. Blood was sampled and placed in heparinized tubes. After centrifugation, plasma was separated into 5 aliquots. One aliquot was considered as the reference aliquot and used immediately for the assay of all of the biochemical constituents. All of the other aliquots were stored at 20 degrees C. Three aliquots underwent 1, 2, or 3 freeze-thaw cycles during a 1- to 3-day period. The last aliquot remained at 20 degrees C throughout the study and was thawed on the third day. The following biochemical constituents were assayed: glucose, urea, creatinine, total proteins, sodium, potassium, chloride, calcium, phosphates, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), and alkaline phosphatase (ALP).

Results: No clinically relevant change was observed between the different aliquots for all of the constituents.

Conclusion: Repeated freeze-thaw cycles do not cause changes in the biochemical constituents studied in canine plasma.