Venezuelan Equine Encephalitis Virus Transmission and Effect on Pathogenesis

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2006 Sep 13

PMID 16965696

Citations 33

Authors

Darci R Smith

Patricia V Aguilar

Lark L Coffey

Gregory D Gromowski

Eryu Wang

Scott C Weaver

Affiliations

Soon will be listed here.

Abstract

Quantifying the dose of an arbovirus transmitted by mosquitoes is essential for designing pathogenesis studies simulating natural infection of vertebrates. Titration of saliva collected in vitro from infected mosquitoes may not accurately estimate titers transmitted during blood feeding, and infection by needle injection may affect vertebrate pathogenesis. We compared the amount of Venezuelan equine encephalitis virus collected from the saliva of Aedes taeniorhynchus to the amount injected into a mouse during blood feeding. Less virus was transmitted by mosquitoes in vivo (geometric mean 11 PFU) than was found for comparable times of salivation in vitro (mean saliva titer 74 PFU). We also observed slightly lower early and late viremia titers in mice that were needle injected with 8 PFU, which represents the low end of the in vivo transmission range. No differences in survival were detected, regardless of the dose or infection route.

Citing Articles

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