Amh and Dmrta2 Genes Map to Tilapia (Oreochromis Spp.) Linkage Group 23 Within Quantitative Trait Locus Regions for Sex Determination

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2006 Sep 5

PMID 16951079

Citations 47

Authors

Andrey Shirak

Eyal Seroussi

Avner Cnaani

Aimee E Howe

Raisa Domokhovsky

Noam Zilberman

Thomas D Kocher

Gideon Hulata

Micha Ron

Affiliations

Soon will be listed here.

Abstract

Recent studies have revealed that the major genes of the mammalian sex determination pathway are also involved in sex determination of fish. Several studies have reported QTL in various species and strains of tilapia, regions contributing to sex determination have been identified on linkage groups 1, 3, and 23. Genes contributing to sex-specific mortality have been detected on linkage groups 2, 6, and 23. To test whether the same genes might control sex determination in mammals and fishes, we mapped 11 genes that are considered putative master key regulators of sex determination: Amh, Cyp19, Dax1, Dmrt2, Dmrta2, Fhl3l, Foxl2, Ixl, Lhx9, Sf1, and Sox8. We identified polymorphisms in noncoding regions of these genes and genotyped these sites for 90 individuals of an F2 mapping family. Mapping of Dax1 joined LG16 and LG21 into a single linkage group. The Amh and Dmrta2 genes were mapped to two distinct regions of LG23. The Amh gene was mapped 5 cM from UNH879 within a QTL region for sex determination and 2 cM from UNH216 within a QTL region for sex-specific mortality. Dmrta2 was mapped 4 cM from UNH848 within another QTL region for sex determination. Cyp19 was mapped to LG1 far from a previously reported QTL region for sex determination on this chromosome. Seven other candidate genes mapped to LG4, -11, -12, -14, and -17.

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References

Lei N, Heckert L . Sp1 and Egr1 regulate transcription of the Dmrt1 gene in Sertoli cells. Biol Reprod. 2002; 66(3):675-84. PMC: 1496933. DOI: 10.1095/biolreprod66.3.675. View

Agnese J, Abban E, Fermon Y . Genetic differentiation among natural populations of the Nile tilapia Oreochromis niloticus (Teleostei, cichlidae). Heredity (Edinb). 1997; 79 ( Pt 1):88-96. DOI: 10.1038/hdy.1997.126. View

Lee B, Hulata G, Kocher T . Two unlinked loci controlling the sex of blue tilapia (Oreochromis aureus). Heredity (Edinb). 2004; 92(6):543-9. DOI: 10.1038/sj.hdy.6800453. View

Zhang J . Evolution of DMY, a newly emergent male sex-determination gene of medaka fish. Genetics. 2004; 166(4):1887-95. PMC: 1470821. DOI: 10.1534/genetics.166.4.1887. View

Koopman P, Loffler K . Sex determination: the fishy tale of Dmrt1. Curr Biol. 2003; 13(5):R177-9. DOI: 10.1016/s0960-9822(03)00117-9. View

DCotta H, Fostier A, Guiguen Y, Govoroun M, Baroiller J . Aromatase plays a key role during normal and temperature-induced sex differentiation of tilapia Oreochromis niloticus. Mol Reprod Dev. 2001; 59(3):265-76. DOI: 10.1002/mrd.1031. View

Nanda I, Shan Z, Schartl M, Burt D, Koehler M, Nothwang H . 300 million years of conserved synteny between chicken Z and human chromosome 9. Nat Genet. 1999; 21(3):258-9. DOI: 10.1038/6769. View

Jurinke C, Van Den Boom D, Cantor C, Koster H . Automated genotyping using the DNA MassArray technology. Methods Mol Biol. 2002; 187:179-92. DOI: 10.1385/1-59259-273-2:179. View

Ottolenghi C, Fellous M, Barbieri M, McElreavey K . Novel paralogy relations among human chromosomes support a link between the phylogeny of doublesex-related genes and the evolution of sex determination. Genomics. 2002; 79(3):333-43. DOI: 10.1006/geno.2002.6711. View

10.

McElreavey K, Vilain E, Abbas N, Herskowitz I, Fellous M . A regulatory cascade hypothesis for mammalian sex determination: SRY represses a negative regulator of male development. Proc Natl Acad Sci U S A. 1993; 90(8):3368-72. PMC: 46301. DOI: 10.1073/pnas.90.8.3368. View

11.

Shirak A, Palti Y, Cnaani A, Korol A, Hulata G, Ron M . Association between loci with deleterious alleles and distorted sex ratios in an inbred line of tilapia (Oreochromis aureus). J Hered. 2002; 93(4):270-6. DOI: 10.1093/jhered/93.4.270. View

12.

Western P, Sinclair A . Sex, genes, and heat: triggers of diversity. J Exp Zool. 2001; 290(6):624-31. DOI: 10.1002/jez.1113. View

13.

Murdock C, Wibbels T . Expression of Dmrt1 in a turtle with temperature-dependent sex determination. Cytogenet Genome Res. 2003; 101(3-4):302-8. DOI: 10.1159/000074353. View

14.

Guo Y, Cheng H, Huang X, Gao S, Yu H, Zhou R . Gene structure, multiple alternative splicing, and expression in gonads of zebrafish Dmrt1. Biochem Biophys Res Commun. 2005; 330(3):950-7. DOI: 10.1016/j.bbrc.2005.03.066. View

15.

Volff J, Schartl M . Variability of genetic sex determination in poeciliid fishes. Genetica. 2002; 111(1-3):101-10. DOI: 10.1023/a:1013795415808. View

16.

Rodriguez-Mari A, Yan Y, Bremiller R, Wilson C, Canestro C, Postlethwait J . Characterization and expression pattern of zebrafish Anti-Müllerian hormone (Amh) relative to sox9a, sox9b, and cyp19a1a, during gonad development. Gene Expr Patterns. 2005; 5(5):655-67. DOI: 10.1016/j.modgep.2005.02.008. View

17.

Hett A, Pitra C, Jenneckens I, Ludwig A . Characterization of Sox9 in European Atlantic sturgeon (Acipenser sturio). J Hered. 2005; 96(2):150-4. DOI: 10.1093/jhered/esi008. View

18.

von Hofsten J, Olsson P . Zebrafish sex determination and differentiation: involvement of FTZ-F1 genes. Reprod Biol Endocrinol. 2005; 3:63. PMC: 1298332. DOI: 10.1186/1477-7827-3-63. View

19.

Uchida D, Yamashita M, Kitano T, Iguchi T . Oocyte apoptosis during the transition from ovary-like tissue to testes during sex differentiation of juvenile zebrafish. J Exp Biol. 2002; 205(Pt 6):711-8. DOI: 10.1242/jeb.205.6.711. View

20.

Valenzuela N, Adams D, Janzen F . Pattern does not equal process: exactly when is sex environmentally determined?. Am Nat. 2003; 161(4):676-83. DOI: 10.1086/368292. View