Arachidonic Acid Regulates the Binding of Human Interferon in Human Skin Fibroblasts

Overview

Journal Lipids

Specialty Biochemistry

Date 1990 Jun 1

PMID 1694948

Citations 2

Authors

S D Menon

W H Yap

A Lim

Y H Tan

Affiliations

Soon will be listed here.

Abstract

The induction of the antiviral state in human fibroblasts by human interferon is inhibited by arachidonic acid, its analogues 5,8,11,14-eicosatetraynoic and 5,8,11-eicosatriynoic acids, as well as by sodium arachidonate. The fatty acids myristic or oleic acid and sodium palmitate do not inhibit the antiviral action of interferon. Experiments were conducted to investigate the mechanism by which arachidonic acid could inhibit the action of interferon. No correlation between cellular lipoxygenase activities and the inhibition of antiviral action of interferon was observed in the fatty acid treated cells. Likewise, the cyclooxygenase inhibitors indomethacin and oxyphenylbutazone do not inhibit the interferon-induced antiviral state. Taken together, the inhibition of interferon action by arachidonates is unlikely to be mediated by cyclooxygenase or lipoxygenase-generated intermediates, even though arachidonates are known to affect the activity of these enzymes in vitro. Measurement of interferon receptors in the fatty acid treated cells showed that arachidonic acid, sodium arachidonate and its analogues decreased the number of human type I interferon receptors available for binding, and inhibited the transcription of the interferon-induced 6-16 gene and the induction of cellular (2'-5')-oligoadenylate synthetase, suggesting the mechanism of inhibition is mediated at the level of the interferon receptor. The significance of the finding that arachidonic acid, a common fatty acid of cells and serum, can affect the antiviral action of interferon is discussed.

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References

Powell W . Precolumn extraction and reversed-phase high-pressure liquid chromatography of prostaglandins and leukotrienes. Anal Biochem. 1987; 164(1):117-31. DOI: 10.1016/0003-2697(87)90375-7. View

ZWINGELSTEIN G, Brichon G, Meister R, Maury C, GRESSER I . Interferon alpha/beta induces changes in the metabolism of polyenoic phospholipids and diacylglycerols in the livers of suckling mice. Lipids. 1987; 22(10):736-43. DOI: 10.1007/BF02533974. View

Hamberg M, Svensson J, Samuelsson B . Prostaglandin endoperoxides. A new concept concerning the mode of action and release of prostaglandins. Proc Natl Acad Sci U S A. 1974; 71(10):3824-8. PMC: 434276. DOI: 10.1073/pnas.71.10.3824. View

Salmon J, Flower R . Extraction and thin-layer chromatography of arachidonic acid metabolites. Methods Enzymol. 1982; 86:477-93. DOI: 10.1016/0076-6879(82)86219-8. View

Exley R, Gordon J, Clemens M . Induction of B-cell differentiation antigens in interferon- or phorbol ester-treated Daudi cells is impaired by inhibitors of ADP-ribosyltransferase. Proc Natl Acad Sci U S A. 1987; 84(18):6467-70. PMC: 299098. DOI: 10.1073/pnas.84.18.6467. View

Russell J, Torres B, Johnson H . Phospholipase A2 treatment of lymphocytes provides helper signal for interferon-gamma induction. Evidence for second messenger role of endogenous arachidonic acid. J Immunol. 1987; 139(10):3442-6. View

Fan X, Goldberg M, Bloom B . Interferon-gamma-induced transcriptional activation is mediated by protein kinase C. Proc Natl Acad Sci U S A. 1988; 85(14):5122-5. PMC: 281700. DOI: 10.1073/pnas.85.14.5122. View

Blomhoff H, Ruud E, Funderud S, Godal T . Distinct effect of forskolin and interferon-gamma on cell proliferation and regulation of histocompatibility antigen expression in hematopoietic cells. Biochim Biophys Acta. 1986; 887(2):150-6. DOI: 10.1016/0167-4889(86)90049-2. View

Pfeffer L, Wang E, Landsberger F, Tamm I . Assays to measure plasma membrane and cytoskeletal changes in interferon-treated cells. Methods Enzymol. 1981; 79(Pt B):461-73. DOI: 10.1016/s0076-6879(81)79060-8. View

10.

Tan U . Chromosome-21-dosage effect on inducibility of anti-viral gene(s). Nature. 1975; 253(5489):280-2. DOI: 10.1038/253280a0. View

11.

Carney D, Scott D, Gordon E, LaBelle E . Phosphoinositides in mitogenesis: neomycin inhibits thrombin-stimulated phosphoinositide turnover and initiation of cell proliferation. Cell. 1985; 42(2):479-88. DOI: 10.1016/0092-8674(85)90105-9. View

12.

Eldor A, Fridman R, Vlodavsky I, Fuks Z, Panet A . Interferon enhances prostacyclin production by cultured vascular endothelial cells. J Clin Invest. 1984; 73(1):251-7. PMC: 425007. DOI: 10.1172/JCI111198. View

13.

Faltynek C, Princler G, Gusella G, Varesio L, Radzioch D . A functional protein kinase C is required for induction of 2-5A synthetase by recombinant interferon-alpha A in Daudi cells. J Biol Chem. 1989; 264(24):14305-11. View

14.

Hammarstrom S . Selective inhibition of platelet n-8 lipoxygenase by 5,8,11-eicosatriynoic acid. Biochim Biophys Acta. 1977; 487(3):517-9. DOI: 10.1016/0005-2760(77)90221-1. View

15.

Friedman R, Manly S, McMahon M, Kerr I, Stark G . Transcriptional and posttranscriptional regulation of interferon-induced gene expression in human cells. Cell. 1984; 38(3):745-55. DOI: 10.1016/0092-8674(84)90270-8. View

16.

Michell B . Oncogenes and inositol lipids. Nature. 1984; 308(5962):770. DOI: 10.1038/308770a0. View

17.

Chandrabose K, Cuatrecasas P, Pottathil R . Changes in fatty acyl chains of phospholipids induced by interferon in mouse sarcoma S-180 cells. Biochem Biophys Res Commun. 1981; 98(3):661-8. DOI: 10.1016/0006-291x(81)91165-7. View

18.

Horiguchi J, Spriggs D, Imamura K, Stone R, Luebbers R, Kufe D . Role of arachidonic acid metabolism in transcriptional induction of tumor necrosis factor gene expression by phorbol ester. Mol Cell Biol. 1989; 9(1):252-8. PMC: 362167. DOI: 10.1128/mcb.9.1.252-258.1989. View

19.

Epstein C, McManus N, Epstein L, BRANCA A, DAlessandro S, Baglioni C . Direct evidence that the gene product of the human chromosome 21 locus, IFRC, is the interferon-alpha receptor. Biochem Biophys Res Commun. 1982; 107(3):1060-6. DOI: 10.1016/0006-291x(82)90629-5. View

20.

Ho M, Kono Y . EFFECT OF ACTINOMYCIN D ON VIRUS AND ENDOTOXIN-INDUCED INTERFERONLIKE INHIBITORS IN RABBITS. Proc Natl Acad Sci U S A. 1965; 53:220-4. PMC: 219458. DOI: 10.1073/pnas.53.1.220. View