Heterogeneity Amongst Splenic Stromal Cell Lines Which Support Dendritic Cell Hematopoiesis

Overview

Journal In Vitro Cell Dev Biol Anim

Publisher Springer

Specialties Biology
Cell Biology

Date 2006 Sep 5

PMID 16948502

Citations 10

Authors

Genevieve Despars

Helen C ONeill

Affiliations

Soon will be listed here.

Abstract

Long-term cultures (LTC) producing dendritic cells (DC) have been previously established from spleen. LTC support the development of nonadherent cells comprising small DC progenitors and immature DC. Similarly, the splenic stroma STX3, derived from a LTC which ceased DC production, can support DC development from precursors in overlaid bone marrow. The STX3 stroma is an immortalised mixed population of endothelial cells and elongated spindle-shaped cells, thought to be fibroblasts. The stromal cell components of STX3 have been studied here. A panel of 102 cell lines was established by single-cell sorting. A range of clone morphology, including cobblestone cells and elongated spindle-shaped cells, was reflective of heterogeneity in STX3. However, similar expression levels for the endothelial genes ACVRL1/ ALK1, COL18A1, and MCAM in 13 splenic stromal cell lines suggested that both cell types had endothelial origin. The hematopoietic support function of stromal clones was tested in coculture assays with a bone marrow cell overlay. Splenic stromal cell lines with different morphology were both supporters and nonsupporters of hematopoiesis, in terms of foci formation or release of suspension cells. Cloning of STX3 led to the isolation of a panel of splenic endothelial cell lines heterogeneous in terms of morphology and hematopoietic support function.

Citing Articles

Skeletal stem/progenitor cells provide the niche for extramedullary hematopoiesis in spleen.

ONeill H, Lim H Front Physiol. 2023; 14:1148414.

PMID: 37007998 PMC: 10063897. DOI: 10.3389/fphys.2023.1148414.

Extramedullary hematopoiesis: mesenchymal stromal cells from spleen provide an in vitro niche for myelopoiesis.

Petvises S, Tran V, Hey Y, Talaulikar D, ONeill T, Tan J In Vitro Cell Dev Biol Anim. 2022; 58(5):429-439.

PMID: 35641778 PMC: 9213314. DOI: 10.1007/s11626-022-00693-8.

Role of SVEP1 in Stroma-Dependent Hematopoiesis .

Tran V, ONeill H Front Cell Dev Biol. 2022; 9:760480.

PMID: 35174156 PMC: 8841349. DOI: 10.3389/fcell.2021.760480.

Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis.

ONeill H, Lim H, Periasamy P, Kumarappan L, Tan J, ONeill T PLoS One. 2019; 14(10):e0223416.

PMID: 31584977 PMC: 6777786. DOI: 10.1371/journal.pone.0223416.

Identification of Stromal Cells in Spleen Which Support Myelopoiesis.

Lim H, ONeill H Front Cell Dev Biol. 2019; 7:1.

PMID: 30733944 PMC: 6354566. DOI: 10.3389/fcell.2019.00001.

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