The Suppressor of Killer of Prune, a Unique Glutathione S-transferase

Overview

Journal J Bioenerg Biomembr

Publisher Springer

Specialties Biochemistry
Biology
Endocrinology

Date 2006 Sep 1

PMID 16944302

Citations 2

Authors

Elayne Provost

Allen Shearn

Affiliations

Soon will be listed here.

Abstract

The prune-Killer of prune conditional dominant, lethal interaction in Drosophila was identified in the 1950s, but its mechanism remains unknown. We undertook a genetic screen for suppressors of this lethal interaction and identified a gene we named, Suppressor of Killer of prune Su(Kpn). Su(Kpn) is a unique protein with four N-terminal FLYWCH zinc-finger domains, an acidic domain and a C-terminal glutathione S-transferase (GST) domain. The GST domain of Su(Kpn) is of particular interest because GSTs are usually independent of other protein domains. While GSTs are generally thought of as detoxifying enzymes, they are also associated with cellular toxicity. We predict that the GST domain of the Su(Kpn) creates a toxic product in prune-Killer of prune flies that is lethal. The substrate of the Su(Kpn) remains unknown.

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References

Steeg P, Bevilacqua G, Pozzatti R, Liotta L, Sobel M . Altered expression of NM23, a gene associated with low tumor metastatic potential, during adenovirus 2 Ela inhibition of experimental metastasis. Cancer Res. 1988; 48(22):6550-4. View

Lascu I, Chaffotte A, Veron M . A Pro/Ser substitution in nucleoside diphosphate kinase of Drosophila melanogaster (mutation killer of prune) affects stability but not catalytic efficiency of the enzyme. J Biol Chem. 1992; 267(18):12775-81. View

Lacombe M, Milon L, Munier A, Mehus J, Lambeth D . The human Nm23/nucleoside diphosphate kinases. J Bioenerg Biomembr. 2002; 32(3):247-58. DOI: 10.1023/a:1005584929050. View

Timmons L, Xu J, Hersperger G, Deng X, Shearn A . Point mutations in awdKpn which revert the prune/Killer of prune lethal interaction affect conserved residues that are involved in nucleoside diphosphate kinase substrate binding and catalysis. J Biol Chem. 1995; 270(39):23021-30. DOI: 10.1074/jbc.270.39.23021. View

STURTEVANT A . A Highly Specific Complementary Lethal System in Drosophila Melanogaster. Genetics. 1956; 41(1):118-23. PMC: 1209758. DOI: 10.1093/genetics/41.1.118. View

Xu J, Liu L, Deng X, Timmons L, Hersperger E, Steeg P . The enzymatic activity of Drosophila AWD/NDP kinase is necessary but not sufficient for its biological function. Dev Biol. 1996; 177(2):544-57. DOI: 10.1006/dbio.1996.0184. View

Timmons L, Shearn A . Role of AWD/nucleoside diphosphate kinase in Drosophila development. J Bioenerg Biomembr. 2002; 32(3):293-300. DOI: 10.1023/a:1005545214937. View

Reymond A, Volorio S, Merla G, Zuffardi O, Bulfone A, Ballabio A . Evidence for interaction between human PRUNE and nm23-H1 NDPKinase. Oncogene. 1999; 18(51):7244-52. DOI: 10.1038/sj.onc.1203140. View

Krauss V, Dorn R . Evolution of the trans-splicing Drosophila locus mod(mdg4) in several species of Diptera and Lepidoptera. Gene. 2004; 331:165-76. DOI: 10.1016/j.gene.2004.02.019. View

10.

Parker S, Parker M, Sah R, Sallee F . Angiogenesis and rhodopsin-like receptors: a role for N-terminal acidic residues?. Biochem Biophys Res Commun. 2005; 335(4):983-92. DOI: 10.1016/j.bbrc.2005.06.158. View

11.

Lee J, Rieu P, Arnaout M, Liddington R . Crystal structure of the A domain from the alpha subunit of integrin CR3 (CD11b/CD18). Cell. 1995; 80(4):631-8. DOI: 10.1016/0092-8674(95)90517-0. View

12.

Dai M, Sun X, Qin J, Smolik S, Lu H . Identification and characterization of a novel Drosophila melanogaster glutathione S-transferase-containing FLYWCH zinc finger protein. Gene. 2004; 342(1):49-56. DOI: 10.1016/j.gene.2004.07.043. View

13.

Ranson H, Rossiter L, Ortelli F, Jensen B, Wang X, Roth C . Identification of a novel class of insect glutathione S-transferases involved in resistance to DDT in the malaria vector Anopheles gambiae. Biochem J. 2001; 359(Pt 2):295-304. PMC: 1222147. DOI: 10.1042/0264-6021:3590295. View

14.

Clayton J, Cripps R, Sparrow J, Bullard B . Interaction of troponin-H and glutathione S-transferase-2 in the indirect flight muscles of Drosophila melanogaster. J Muscle Res Cell Motil. 1998; 19(2):117-27. DOI: 10.1023/a:1005304527563. View

15.

Rubin G, Yandell M, Wortman J, Gabor Miklos G, NELSON C, Hariharan I . Comparative genomics of the eukaryotes. Science. 2000; 287(5461):2204-15. PMC: 2754258. DOI: 10.1126/science.287.5461.2204. View

16.

Buchner K, Roth P, Schotta G, Krauss V, Saumweber H, Reuter G . Genetic and molecular complexity of the position effect variegation modifier mod(mdg4) in Drosophila. Genetics. 2000; 155(1):141-57. PMC: 1461079. DOI: 10.1093/genetics/155.1.141. View

17.

Rosengard A, Krutzsch H, Shearn A, Biggs J, Barker E, Margulies I . Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development. Nature. 1989; 342(6246):177-80. DOI: 10.1038/342177a0. View

18.

Sawicki R, Singh S, Mondal A, Benes H, Zimniak P . Cloning, expression and biochemical characterization of one Epsilon-class (GST-3) and ten Delta-class (GST-1) glutathione S-transferases from Drosophila melanogaster, and identification of additional nine members of the Epsilon class. Biochem J. 2002; 370(Pt 2):661-9. PMC: 1223192. DOI: 10.1042/BJ20021287. View

19.

Pickett C, Lu A . Glutathione S-transferases: gene structure, regulation, and biological function. Annu Rev Biochem. 1989; 58:743-64. DOI: 10.1146/annurev.bi.58.070189.003523. View

20.

Biggs J, Tripoulas N, Hersperger E, Dearolf C, Shearn A . Analysis of the lethal interaction between the prune and Killer of prune mutations of Drosophila. Genes Dev. 1988; 2(10):1333-43. DOI: 10.1101/gad.2.10.1333. View