Aflatoxigenicity in Aspergillus: Molecular Genetics, Phylogenetic Relationships and Evolutionary Implications

Overview

Journal Mycopathologia

Specialties Microbiology
Pharmacology

Date 2006 Sep 1

PMID 16944284

Citations 27

Authors

Jeffrey W Cary

Kenneth C Ehrlich

Affiliations

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Abstract

Aflatoxins (AFs) are toxic and carcinogenic secondary metabolites produced by isolates of Aspergillus section Flavi as well as a number of Aspergillus isolates that are classified outside of section Flavi. Characterization of the AF and sterigmatocystin (ST) gene clusters and analysis of factors governing regulation of their biosynthesis has resulted in these two mycotoxins being the most extensively studied of fungal secondary metabolites. This wealth of information has allowed the determination of the molecular basis for non-production of AF in natural isolates of A. flavus and domesticated strains of A. oryzae. This review provides an overview of the molecular analysis of the AF and ST gene clusters as well as new information on an AF gene cluster identified in the non-section Flavi isolate, Aspergillus ochraceoroseus. Additionally, molecular phylogenetic analysis using AF biosynthetic gene sequences as well as ribosomal DNA internal transcribed spacer (ITS) sequences between various section Flavi and non-section Flavi species has enabled determination of the probable evolutionary history of the AF and ST gene clusters. A model for the evolution of the AF and ST gene clusters as well as possible biological roles for AF are discussed.

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References

Narendja F, Davis M, Hynes M . AnCF, the CCAAT binding complex of Aspergillus nidulans, is essential for the formation of a DNase I-hypersensitive site in the 5' region of the amdS gene. Mol Cell Biol. 1999; 19(10):6523-31. PMC: 84622. DOI: 10.1128/MCB.19.10.6523. View

Muro-Pastor M, Gonzalez R, Strauss J, Narendja F, Scazzocchio C . The GATA factor AreA is essential for chromatin remodelling in a eukaryotic bidirectional promoter. EMBO J. 1999; 18(6):1584-97. PMC: 1171246. DOI: 10.1093/emboj/18.6.1584. View

Cary J, Klich M, Beltz S . Characterization of aflatoxin-producing fungi outside of Aspergillus section Flavi. Mycologia. 2006; 97(2):425-32. DOI: 10.3852/mycologia.97.2.425. View

Pringle A, Taylor J . The fitness of filamentous fungi. Trends Microbiol. 2002; 10(10):474-81. DOI: 10.1016/s0966-842x(02)02447-2. View

Boyd M, Cotty P . Aspergillus flavus and Aflatoxin Contamination of Leguminous Trees of the Sonoran Desert in Arizona. Phytopathology. 2008; 91(9):913-9. DOI: 10.1094/PHYTO.2001.91.9.913. View

Inagaki A, Takano Y, Kubo Y, Mise K, Furusawa I . Construction of an equalized cDNA library from Colletotrichum lagenarium and its application to the isolation of differentially expressed genes. Can J Microbiol. 2000; 46(2):150-8. DOI: 10.1139/w99-119. View

Heckman D, Geiser D, Eidell B, STAUFFER R, Kardos N, Hedges S . Molecular evidence for the early colonization of land by fungi and plants. Science. 2001; 293(5532):1129-33. DOI: 10.1126/science.1061457. View

Henson J, Butler M, Day A . THE DARK SIDE OF THE MYCELIUM: Melanins of Phytopathogenic Fungi. Annu Rev Phytopathol. 2001; 37:447-471. DOI: 10.1146/annurev.phyto.37.1.447. View

Yu J, Chang P, Bhatnagar D, Cleveland T . Cloning of a sugar utilization gene cluster in Aspergillus parasiticus. Biochim Biophys Acta. 2000; 1493(1-2):211-4. DOI: 10.1016/s0167-4781(00)00148-2. View

10.

Wilkinson H, Ramaswamy A, Sim S, Keller N . Increased conidiation associated with progression along the sterigmatocystin biosynthetic pathway. Mycologia. 2010; 96(6):1190-8. View

11.

Yu J, Chang P, Ehrlich K, Cary J, Bhatnagar D, Cleveland T . Clustered pathway genes in aflatoxin biosynthesis. Appl Environ Microbiol. 2004; 70(3):1253-62. PMC: 368384. DOI: 10.1128/AEM.70.3.1253-1262.2004. View

12.

Han S, Navarro J, Greve R, Adams T . Translational repression of brlA expression prevents premature development in Aspergillus. EMBO J. 1993; 12(6):2449-57. PMC: 413480. DOI: 10.1002/j.1460-2075.1993.tb05899.x. View

13.

Kasuga T, White T, Taylor J . Estimation of nucleotide substitution rates in Eurotiomycete fungi. Mol Biol Evol. 2002; 19(12):2318-24. DOI: 10.1093/oxfordjournals.molbev.a004056. View

14.

Frisvad J, Samson R, Smedsgaard J . Emericella astellata, a new producer of aflatoxin B, B and sterigmatocystin. Lett Appl Microbiol. 2004; 38(5):440-5. DOI: 10.1111/j.1472-765X.2004.01520.x. View

15.

Schmidt S, Sunyaev S, Bork P, Dandekar T . Metabolites: a helping hand for pathway evolution?. Trends Biochem Sci. 2003; 28(6):336-41. DOI: 10.1016/S0968-0004(03)00114-2. View

16.

Frisvad J, Samson R . Emericella venezuelensis, a new species with stellate ascospores producing sterigmatocystin and aflatoxin B1. Syst Appl Microbiol. 2004; 27(6):672-80. DOI: 10.1078/0723202042369910. View

17.

Andrianopoulos A, Timberlake W . The Aspergillus nidulans abaA gene encodes a transcriptional activator that acts as a genetic switch to control development. Mol Cell Biol. 1994; 14(4):2503-15. PMC: 358618. DOI: 10.1128/mcb.14.4.2503-2515.1994. View

18.

Wheeler D, Hope R, Cooper S, Dolman G, Webb G, Bottema C . An orphaned mammalian beta-globin gene of ancient evolutionary origin. Proc Natl Acad Sci U S A. 2001; 98(3):1101-6. PMC: 14715. DOI: 10.1073/pnas.98.3.1101. View

19.

Fujii I, Chen Z, Ebizuka Y, Sankawa U . Identification of emodinanthrone oxygenase in fungus Aspergillus terreus. Biochem Int. 1991; 25(6):1043-9. View

20.

Geiser D, Dorner J, Horn B, Taylor J . The phylogenetics of mycotoxin and sclerotium production in Aspergillus flavus and Aspergillus oryzae. Fungal Genet Biol. 2001; 31(3):169-79. DOI: 10.1006/fgbi.2000.1215. View