Gel-based Hippocampal Proteomic Analysis 2 Weeks Following Traumatic Brain Injury to Immature Rats Using Controlled Cortical Impact

Overview

Journal Dev Neurosci

Specialty Neurology

Date 2006 Sep 1

PMID 16943664

Citations 28

Authors

Ashley R Kochanek

Anthony E Kline

Wei-Min Gao

Mandeep Chadha

Yichen Lai

Robert S B Clark

C Edward Dixon

Larry W Jenkins

Affiliations

Soon will be listed here.

Abstract

Traumatic brain injury (TBI) to postnatal day 17 rats has been shown to produce acute changes in hippocampal global protein levels and spatial learning and memory deficits. The purpose of the present study was to analyze global hippocampal protein changes 2 weeks after a moderate ipsilateral controlled cortical impact in postnatal day 17 rats using 2-dimensional difference gel electrophoresis and mass spectrometry. Paired sham and ipsilateral injured hippocampal lysates were independently labeled with different fluorescent cyanine dyes and coseparated within the same immobilized pH gradient strips and slab gel based on isoelectric point and molecular mass. Significant changes in key proteins involved in glial and neuronal stress, oxidative metabolism, calcium uptake and neurotransmitter function were found 2 weeks after injury, and their potential roles in hippocampal plasticity and cognitive dysfunction were discussed.

Citing Articles

Pediatric Traumatic Brain Injury: Models, Therapeutics, and Outcomes.

Moschonas E, Annas E, Zamudio-Flores J, Jarvis J, Lajud N, Bondi C Adv Neurobiol. 2024; 42:147-163.

PMID: 39432041 DOI: 10.1007/978-3-031-69832-3_7.

Advances in neuroproteomics for neurotrauma: unraveling insights for personalized medicine and future prospects.

Kobeissy F, Goli M, Yadikar H, Shakkour Z, Kurup M, Haidar M Front Neurol. 2023; 14:1288740.

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Mass Spectrometry-Based Approaches for Clinical Biomarker Discovery in Traumatic Brain Injury.

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Pathophysiology of Pediatric Traumatic Brain Injury.

Serpa R, Ferguson L, Larson C, Bailard J, Cooke S, Greco T Front Neurol. 2021; 12:696510.

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Metabolic perturbations after pediatric TBI: It's not just about glucose.

Bowman C, Scafidi J, Scafidi S Exp Neurol. 2019; 316:74-84.

PMID: 30951705 PMC: 6739863. DOI: 10.1016/j.expneurol.2019.03.018.

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