» Articles » PMID: 16936306

Differences Between Human Embryonic Stem Cell Lines

Overview
Date 2006 Aug 29
PMID 16936306
Citations 76
Authors
Affiliations
Soon will be listed here.
Abstract

The promise of human embryonic stem cell (hESC) lines for treating injuries and degenerative diseases, for understanding early human development, for disease modelling and for drug discovery, has brought much excitement to scientific communities as well as to the public. Although all of the lines derived worldwide share the expression of characteristic pluripotency markers, many differences are emerging between lines that may be more associated with the wide range of culture conditions in current use than the inherent genetic variation of the embryos from which embryonic stem cells were derived. Thus, the validity of many comparisons between lines published thus far is difficult to interpret. This article reviews the evidence for differences between lines, focusing on studies of pluripotency marker molecules, transcriptional profiling, genetic stability and epigenetic stability, for which there is most evidence. Recognition and assessment of environmentally induced differences will be important to facilitate the development of culture systems that maximize stability in culture and provide lines with maximal potential for safety and success in the range of possible applications.

Citing Articles

Evaluating Riboglow-FLIM probes for RNA sensing.

Sarfraz N, Shafik L, Stickelman Z, Shankar U, Moscoso E, Braselmann E RSC Chem Biol. 2024; 5(2):109-116.

PMID: 38333191 PMC: 10849122. DOI: 10.1039/d3cb00197k.


Analysis of the effects of bench-scale cell culture platforms and inoculum cell concentrations on PSC aggregate formation and culture.

Iworima D, Baker R, Piret J, Kieffer T Front Bioeng Biotechnol. 2023; 11:1267007.

PMID: 38107616 PMC: 10722899. DOI: 10.3389/fbioe.2023.1267007.


Profiling transcriptomic responses of human stem cell-derived medium spiny neuron-like cells to exogenous phasic and tonic neurotransmitters.

Tam R, Keung A Mol Cell Neurosci. 2023; 126:103876.

PMID: 37385515 PMC: 10528483. DOI: 10.1016/j.mcn.2023.103876.


Quality Control of Human Pluripotent Stem Cell Colonies by Computational Image Analysis Using Convolutional Neural Networks.

Mamaeva A, Krasnova O, Khvorova I, Kozlov K, Gursky V, Samsonova M Int J Mol Sci. 2023; 24(1).

PMID: 36613583 PMC: 9820636. DOI: 10.3390/ijms24010140.


Investigation of murine host sex as a biological variable in epithelial barrier function and muscle contractility in human intestinal organoids.

Beanland B, Mcneill E, Sequeira D, Xue H, Shroyer N, Speer A FASEB J. 2022; 36(11):e22613.

PMID: 36250916 PMC: 9645459. DOI: 10.1096/fj.202101740RR.