Cryo-electron Tomographic Structure of an Immunodeficiency Virus Envelope Complex in Situ

Overview

Journal PLoS Pathog

Specialty Microbiology

Date 2006 Aug 29

PMID 16933990

Citations 123

Authors

Giulia Zanetti

John A G Briggs

Kay Grunewald

Quentin J Sattentau

Stephen D Fuller

Affiliations

Soon will be listed here.

Abstract

The envelope glycoprotein (Env) complexes of the human and simian immunodeficiency viruses (HIV and SIV, respectively) mediate viral entry and are a target for neutralizing antibodies. The receptor binding surfaces of Env are in large part sterically occluded or conformationally masked prior to receptor binding. Knowledge of the unliganded, trimeric Env structure is key for an understanding of viral entry and immune escape, and for the design of vaccines to elicit neutralizing antibodies. We have used cryo-electron tomography and averaging to obtain the structure of the SIV Env complex prior to fusion. Our result reveals novel details of Env organisation, including tight interaction between monomers in the gp41 trimer, associated with a three-lobed, membrane-distal gp120 trimer. A cavity exists at the gp41-gp120 trimer interface. Our model for the spike structure agrees with previously predicted interactions between gp41 monomers, and furthers our understanding of gp120 interactions within an intact spike.

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