Simplified Northern Blot Hybridization Using 5% Sodium Dodecyl Sulfate

Overview

Journal Biotechniques

Specialties Biomedical Engineering
Biotechnology

Date 1990 Apr 1

PMID 1692715

Citations 40

Authors

G D Virca

W Northemann

B R Shiels

G Widera

S Broome

Affiliations

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Abstract

We describe a simplified Northern blot hybridization procedure. This procedure eliminates the need for many reagents commonly used in RNA hybridization and replaces them with two buffers containing 5% sodium dodecyl sulfate for pre-hybridization, hybridization and all post-hybridization washes.

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