Coupling of DNA Replication to Growth Rate in Escherichia Coli: a Possible Role for Guanosine Tetraphosphate

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1990 Apr 1

PMID 1690706

Citations 48

Authors

A E Chiaramello

J W Zyskind

Affiliations

Soon will be listed here.

Abstract

Two promoters for the Escherichia coli operon that contains the four genes dnaA, dnaN, recF, and gyrB were found to be growth rate regulated and under stringent control. Transcript abundance relative to total RNA increased with the growth rate. Changes in transcription from the dnaAp1 and dnaAp2 promoters that were induced by amino acid starvation and chloramphenicol and were relA dependent were correlated with the stringent response. The abundance of these transcripts per total RNA also decreased in spoT mutants as the severity of the mutation increased (guanosine 5'-diphosphate 3'-diphosphate [ppGpp] basal levels increased). Because expression of these promoters appears to be inhibited by ppGpp, it is proposed that one mechanism for coupling DNA replication to the growth rate of bacteria is through ppGpp synthesis at the ribosome.

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References

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

Tosa T, PIZER L . Biochemical bases for the antimetabolite action of L-serine hydroxamate. J Bacteriol. 1971; 106(3):972-82. PMC: 248741. DOI: 10.1128/jb.106.3.972-982.1971. View

Ryals J, Little R, Bremer H . Control of rRNA and tRNA syntheses in Escherichia coli by guanosine tetraphosphate. J Bacteriol. 1982; 151(3):1261-8. PMC: 220404. DOI: 10.1128/jb.151.3.1261-1268.1982. View

Lagosky P, Chang F . Influence of amino acid starvation on guanosine 5'-diphosphate 3'-diphosphate basal-level synthesis in Escherichia coli. J Bacteriol. 1980; 144(2):499-508. PMC: 294696. DOI: 10.1128/jb.144.2.499-508.1980. View

Gourse R, Stark M, Dahlberg A . Regions of DNA involved in the stringent control of plasmid-encoded rRNA in vivo. Cell. 1983; 32(4):1347-54. DOI: 10.1016/0092-8674(83)90315-x. View

Sarmientos P, Sylvester J, Contente S, CASHEL M . Differential stringent control of the tandem E. coli ribosomal RNA promoters from the rrnA operon expressed in vivo in multicopy plasmids. Cell. 1983; 32(4):1337-46. DOI: 10.1016/0092-8674(83)90314-8. View

Hansen F, HANSEN E, Atlung T . The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli. EMBO J. 1982; 1(9):1043-8. PMC: 553160. DOI: 10.1002/j.1460-2075.1982.tb01294.x. View

Adachi T, Mizuuchi K, Menzel R, Gellert M . DNA sequence and transcription of the region upstream of the E. coli gyrB gene. Nucleic Acids Res. 1984; 12(16):6389-95. PMC: 320084. DOI: 10.1093/nar/12.16.6389. View

Pedersen F, Lund E, KJELDGAARD N . Codon specific, tRNA dependent in vitro synthesis of ppGpp and pppGpp. Nat New Biol. 1973; 243(122):13-5. View

10.

DICKSON R, Gaal T, deBoer H, deHaseth P, Gourse R . Identification of promoter mutants defective in growth-rate-dependent regulation of rRNA transcription in Escherichia coli. J Bacteriol. 1989; 171(9):4862-70. PMC: 210290. DOI: 10.1128/jb.171.9.4862-4870.1989. View

11.

McHenry C . DNA polymerase III holoenzyme of Escherichia coli. Annu Rev Biochem. 1988; 57:519-50. DOI: 10.1146/annurev.bi.57.070188.002511. View

12.

Armengod M, Lambies E . Transcriptional organization of the dnaN and recF genes of Escherichia coli K-12. J Biol Chem. 1988; 263(24):12109-14. View

13.

Quinones A, Kaasch J, KAASCH M, Messer W . Induction of dnaN and dnaQ gene expression in Escherichia coli by alkylation damage to DNA. EMBO J. 1989; 8(2):587-93. PMC: 400845. DOI: 10.1002/j.1460-2075.1989.tb03413.x. View

14.

Yamagishi M, Cole J, Nomura M, Studier F, Dunn J . Stringent control in Escherichia coli applies also to transcription by T7 RNA polymerase. J Biol Chem. 1987; 262(9):3940-3. View

15.

Rokeach L, Kassavetis G, Zyskind J . RNA polymerase pauses in vitro within the Escherichia coli origin of replication at the same sites where termination occurs in vivo. J Biol Chem. 1987; 262(15):7264-72. View

16.

Armengod M, Lambies E . Overlapping arrangement of the recF and dnaN operons of Escherichia coli; positive and negative control sequences. Gene. 1986; 43(3):183-96. DOI: 10.1016/0378-1119(86)90206-4. View

17.

Quinones A, Piechocki R, Messer W . Expression of the Escherichia coli dnaQ (mutD) gene is inducible. Mol Gen Genet. 1988; 211(1):106-12. DOI: 10.1007/BF00338400. View

18.

Quinones A, Messer W . Discoordinate gene expression in the dnaA-dnaN operon of Escherichia coli. Mol Gen Genet. 1988; 213(1):118-24. DOI: 10.1007/BF00333407. View

19.

Wang Q, Kaguni J . Transcriptional repression of the dnaA gene of Escherichia coli by dnaA protein. Mol Gen Genet. 1987; 209(3):518-25. DOI: 10.1007/BF00331158. View

20.

Sekimizu K, Kornberg A . Cardiolipin activation of dnaA protein, the initiation protein of replication in Escherichia coli. J Biol Chem. 1988; 263(15):7131-5. View