Optimizing and Characterizing Alignment of Oriented Lipid Bilayers Containing Gramicidin D

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1990 Feb 1

PMID 1690576

Citations 32

Authors

F Moll 3rd

T A Cross

Affiliations

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Abstract

31P NMR spectroscopy and optical microscopy have been used to characterize samples of gramicidin D in oriented lipid bilayers. Correlations have been made between the defect structures observed under crossed polarizers by optical microscopy and characteristic features of 31P NMR spectra. The sample preparation protocol has been improved using these techniques to achieve minimal dispersion of the bilayer normal and minimal amounts of unoriented sample. The molar ratio of gramicidin to dimyristoyl-phosphatidylcholine, the extent of hydration, and the cosolubilizing solvent system were used as the protocol variables. While hydration level and solvent system had profound effects on the sample orientation the molar ratio did not. However, the 31P chemical shift anisotropy is very sensitive to the molar ratio and can be used as an in situ method for determining the molar ratio.

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References

Rothgeb T, Oldfield E . Nitrogen-14 nuclear magnetic resonance spectroscopy as a probe of lipid bilayer headgroup structure. J Biol Chem. 1981; 256(12):6004-9. View

Smith R, Cornell B . The dynamics of the intrinsic membrane polypeptide gramicidin a in phospholipid bilayers: a solid state carbon-13 NMR study. Biophys J. 2009; 49(1):117-8. PMC: 1329601. DOI: 10.1016/S0006-3495(86)83617-7. View

Thayer A, Kohler S . Phosphorus-31 nuclear magnetic resonance spectra characteristic of hexagonal and isotropic phospholipid phases generated from phosphatidylethanolamine in the bilayer phase. Biochemistry. 1981; 20(24):6831-4. DOI: 10.1021/bi00527a014. View

Cavatorta P, Spisni A, Casali E, Lindner L, Masotti L, Urry D . Intermolecular interactions of gramicidin A' transmembrane channels incorporated into lysophosphatidylcholine lipid systems. Biochim Biophys Acta. 1982; 689(1):113-20. DOI: 10.1016/0005-2736(82)90195-x. View

Masotti L, Spisni A, Urry D . Conformational studies on the gramicidin A transmembrane channel in lipid micelles and liposomes. Cell Biophys. 1980; 2(3):241-51. DOI: 10.1007/BF02790452. View

Spisni A, Pasquali-Ronchetti I, Casali E, Lindner L, Cavatorta P, Masotti L . Supramolecular organization of lysophosphatidylcholine-packaged Gramicidin A. Biochim Biophys Acta. 1983; 732(1):58-68. DOI: 10.1016/0005-2736(83)90186-4. View

van de Ven M, Kattenberg M, van Ginkel G, Verwer W, Levine Y . Angle resolved depolarised resonance Raman studies of beta-carotene in lipid/water mixtures. Biochem Biophys Res Commun. 1984; 120(3):1060-6. DOI: 10.1016/s0006-291x(84)80214-4. View

Engelman D, Zaccai G . Bacteriorhodopsin is an inside-out protein. Proc Natl Acad Sci U S A. 1980; 77(10):5894-8. PMC: 350178. DOI: 10.1073/pnas.77.10.5894. View

Clark N, Rothschild K, Luippold D, Simon B . Surface-induced lamellar orientation of multilayer membrane arrays. Theoretical analysis and a new method with application to purple membrane fragments. Biophys J. 1980; 31(1):65-96. PMC: 1328765. DOI: 10.1016/S0006-3495(80)85041-7. View

10.

Cullis P, de Kruijff B . Lipid polymorphism and the functional roles of lipids in biological membranes. Biochim Biophys Acta. 1979; 559(4):399-420. DOI: 10.1016/0304-4157(79)90012-1. View

11.

Seelig J . 31P nuclear magnetic resonance and the head group structure of phospholipids in membranes. Biochim Biophys Acta. 1978; 515(2):105-40. DOI: 10.1016/0304-4157(78)90001-1. View

12.

Herzfeld J, Griffin R, Haberkorn R . Phosphorus-31 chemical-shift tensors in barium diethyl phosphate and urea-phosphoric acid: model compounds for phospholipid head-group studies. Biochemistry. 1978; 17(14):2711-8. DOI: 10.1021/bi00607a003. View

13.

Seelig J, Gally H . Investigation of phosphatidylethanolamine bilayers by deuterium and phosphorus-31 nuclear magnetic resonance. Biochemistry. 1976; 15(24):5199-204. DOI: 10.1021/bi00669a001. View

14.

Kohler S, Klein M . Orientation and dynamics of phospholipid head groups in bilayers and membranes determined from 31P nuclear magnetic resonance chemical shielding tensors. Biochemistry. 1977; 16(3):519-26. DOI: 10.1021/bi00622a028. View

15.

Saibil H, Chabre M . Orientation of rhodopsin alpha-helices in in retinal rod outer segment membranes studied by infrared linear dichroism. Proc Natl Acad Sci U S A. 1979; 76(9):4405-8. PMC: 411583. DOI: 10.1073/pnas.76.9.4405. View

16.

Herbette L, Marquardt J, Scarpa A, Blasie J . A direct analysis of lamellar x-ray diffraction from hydrated oriented multilayers of fully functional sarcoplasmic reticulum. Biophys J. 1977; 20(2):245-72. PMC: 1473386. DOI: 10.1016/S0006-3495(77)85547-1. View

17.

Asher S, Pershan P . Alignment and defect structures in oriented phosphatidylcholine multilayers. Biophys J. 1979; 27(3):393-421. PMC: 1328596. DOI: 10.1016/S0006-3495(79)85225-X. View

18.

Buldt G, Gally H, Seelig J, Zaccai G . Neutron diffraction studies on phosphatidylcholine model membranes. I. Head group conformation. J Mol Biol. 1979; 134(4):673-91. DOI: 10.1016/0022-2836(79)90479-0. View

19.

Powers L, Pershan P . Monodomain samples of dipalmitoyl phosphatidylcholine with varying concentrations of water and other ingredients. Biophys J. 1977; 20(2):137-52. PMC: 1473390. DOI: 10.1016/S0006-3495(77)85540-9. View

20.

Neugebauer D, Blaurock A . Magnetic orientation of purple membranes demonstrated by optical measurements and neutron scattering. FEBS Lett. 1977; 78(1):31-5. DOI: 10.1016/0014-5793(77)80266-4. View