Protein Translocation by Bacterial Toxin Channels: a Comparison of Diphtheria Toxin and Colicin Ia

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2006 Aug 15

PMID 16905612

Citations 6

Authors

Zhengyan Wu

Karen S Jakes

Ben S Samelson-Jones

Bing Lai

Gang Zhao

Erwin London

Alan Finkelstein

Affiliations

Soon will be listed here.

Abstract

Regions of both colicin Ia and diphtheria toxin N-terminal to the channel-forming domains can be translocated across planar phospholipid bilayer membranes. In this article we show that the translocation pathway of diphtheria toxin allows much larger molecules to be translocated than does the translocation pathway of colicin Ia. In particular, the folded A chain of diphtheria toxin is readily translocated by that toxin but is not translocated by colicin Ia. This difference cannot be attributed to specific recognition of the A chain by diphtheria toxin's translocation pathway because the translocation pathway also accommodates folded myoglobin.

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