Caenorhabditis Elegans MAI-1 Protein, Which is Similar to Mitochondrial ATPase Inhibitor (IF1), Can Inhibit Yeast F0F1-ATPase but Cannot Be Transported to Yeast Mitochondria

Overview

Journal J Bioenerg Biomembr

Publisher Springer

Specialties Biochemistry
Biology
Endocrinology

Date 2006 Aug 10

PMID 16897438

Citations 8

Authors

Naoki Ichikawa

Chiyoko Ando

Mina Fumino

Affiliations

Soon will be listed here.

Abstract

In Caenorhabditis elegans, two proteins that are similar to mitochondrial ATPase inhibitor protein (IF(1)) have been found and named MAI-1 and MAI-2. In this study, we overexpressed and purified both the proteins and examined their properties. Circular dichroism spectra indicated that both the MAI-1 and MAI-2 predominantly consisted of beta- and random structure, and in contrast to mammalian IF(1), alpha-helixes were barely detected. Both MAI-1 and MAI-2 could inhibit yeast F(0)F(1)-ATPase, but the inhibition by MAI-1 was pH-independent. MAI-2-GFP fusion protein was transported to yeast mitochondria, but MAI-1-GFP was not. These results indicate that the MAI-2 is (C. elegans) IF(1). MAI-1 seems to be a cytosolic protein and may regulate cytosolic ATPase(s).

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