Free N-linked Oligosaccharide Chains: Formation and Degradation

Overview

Journal Glycoconj J

Publisher Springer

Specialties Biochemistry
Endocrinology

Date 2006 Aug 10

PMID 16897173

Citations 29

Authors

Tadashi Suzuki

Yoko Funakoshi

Affiliations

Soon will be listed here.

Abstract

There is growing evidence that N-linked glycans play pivotal roles in protein folding and intra- and/or intercellular trafficking of N-glycosylated proteins. It has been shown that during the N-glycosylation of proteins, significant amounts of free oligosaccharides (free OSs) are generated in the lumen of the endoplasmic reticulum (ER) by a mechanism which remains to be clarified. Free OSs are also formed in the cytosol by enzymatic deglycosylation of misfolded glycoproteins, which are subjected to destruction by a cellular system called "ER-associated degradation (ERAD)." While the precise functions of free OSs remain obscure, biochemical studies have revealed that a novel cellular process enables them to be catabolized in a specialized manner, that involves pumping free OSs in the lumen of the ER into the cytosol where further processing occurs. This process is followed by entry into the lysosomes. In this review we summarize current knowledge about the formation, processing and degradation of free OSs in eukaryotes and also discuss the potential biological significance of this pathway. Other evidence for the occurrence of free OSs in various cellular processes is also presented.

Citing Articles

Comprehensive Analysis of the Structure and Function of Peptide:N-Glycanase 1 and Relationship with Congenital Disorder of Deglycosylation.

Miao X, Wu J, Chen H, Lu G Nutrients. 2022; 14(9).

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NGLY1 Deficiency, a Congenital Disorder of Deglycosylation: From Disease Gene Function to Pathophysiology.

Pandey A, Adams J, Han S, Jafar-Nejad H Cells. 2022; 11(7).

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Purification, Characterization, and Gene Expression of Rice Endo-β--Acetylglucosaminidase, Endo-Os.

Maeda M, Okamoto N, Araki N, Kimura Y Front Plant Sci. 2021; 12:647684.

PMID: 34447396 PMC: 8382983. DOI: 10.3389/fpls.2021.647684.

Cytosolic Free -Glycans Are Retro-Transported Into the Endoplasmic Reticulum in Plant Cells.

Katsube M, Ebara N, Maeda M, Kimura Y Front Plant Sci. 2021; 11:610124.

PMID: 33537045 PMC: 7847903. DOI: 10.3389/fpls.2020.610124.

Free glycans derived from -mannosylated glycoproteins suggest the presence of an -glycoprotein degradation pathway in yeast.

Hirayama H, Matsuda T, Tsuchiya Y, Oka R, Seino J, Huang C J Biol Chem. 2019; 294(44):15900-15911.

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