Molecular Cloning and Differential Expression of an Gamma-aminobutyrate Transaminase Gene, OsGABA-T, in Rice (Oryza Sativa) Leaves Infected with Blast Fungus

Overview

Journal J Plant Res

Specialty Biology

Date 2006 Aug 10

PMID 16896530

Citations 8

Authors

Chunxia Wu

Shanyue Zhou

Quan Zhang

Wensheng Zhao

Youliang Peng

Affiliations

Soon will be listed here.

Abstract

gamma-Aminobutyrate transaminase (GABA-T) catalyzes the conversion of GABA to succinic semialdehyde. Using differential display PCR and cDNA library screening, a full-length GABA-T cDNA (OsGABA-T) was isolated from rice (Oryza sativa) leaves infected with an incompatible race of Magnaporthe grisea. The deduced amino acid sequence comprises 483 amino acid residues and shares 85-69% identity with GABA-T sequences from other plants. OsGABA-T expression is induced by blast fungus infection, mechanical wounding and ultraviolet radiation in rice leaves and is not detected in normal rice organs. This gene is also induced by defense signal molecules such as salicylic acid and abscisic acid, but not by jasmonic acid. Our data suggest that OsGABA-T (GABA shunt) may play a role in restricting the levels of cell death during the host-pathogen interaction.

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