Solvent Proton Relaxation of Aqueous Solutions of the Serum Proteins Alpha 2-macroglobulin, Fibrinogen, and Albumin

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1990 Mar 1

PMID 1689591

Citations 3

Authors

R S Menon

P S Allen

Affiliations

Soon will be listed here.

Abstract

The longitudinal, transverse, and spin-locked rotating frame relaxation rates have been measured for water protons in aqueous solutions of the human serum proteins albumin, fibrinogen, and alpha 2-macroglobulin in the physiological concentration range below 50 g/liter, corresponding to an upper limit for molarity of 725, 147, and 69 microM, respectively. The linear concentration dependence of all the relaxation rates measured at 100 MHz was used to provide the molar sensitivities of each relaxation process for each of the protein solutes. Both the solute dependence and the relaxation-process dependence of the molar sensitivities have been analyzed in terms of a model that has emerged from previous R1 dispersion measurements. This analysis demonstrates consistency between our data and that model for the active motions and their motional rates.

Citing Articles

R1ρ sensitivity to pH and other compounds at clinically accessible spin-lock fields in the presence of proteins.

Owusu N, Johnson C, Kearney W, Thedens D, Wemmie J, Magnotta V NMR Biomed. 2019; 33(2):e4217.

PMID: 31742802 PMC: 7043777. DOI: 10.1002/nbm.4217.

Magnetic resonance water proton relaxation in protein solutions and tissue: T(1rho) dispersion characterization.

Chen E, Kim R PLoS One. 2010; 5(1):e8565.

PMID: 20052404 PMC: 2797329. DOI: 10.1371/journal.pone.0008565.

Relaxation rates of blood with osmotically modified red cell volume: application of the two-compartment fast exchange model.

Yu O, Mauss Y, Eclancher B MAGMA. 1999; 7(1):35-41.

PMID: 9877458 DOI: 10.1007/BF02592255.

References

Burnell E, Clark M, HINKE J, Chapman N . Water in barnacle muscle. III. NMR studies of fresh fibers and membrane-damaged fibers equilibrated with selected solutes. Biophys J. 1981; 33(1):1-26. PMC: 1327394. DOI: 10.1016/S0006-3495(81)84869-2. View

Bryant R, Shirley W . Dynamical deductions from nuclear magnetic resonance relaxation measurements at the water-protein interface. Biophys J. 1980; 32(1):3-16. PMC: 1327249. DOI: 10.1016/S0006-3495(80)84912-5. View

Kang Y, Gore J, Armitage I . Studies of factors affecting the design of NMR contrast agents: manganese in blood as a model system. Magn Reson Med. 1984; 1(3):396-409. DOI: 10.1002/mrm.1910010310. View

Fullerton G, POTTER J, Dornbluth N . NMR relaxation of protons in tissues and other macromolecular water solutions. Magn Reson Imaging. 1982; 1(4):209-26. DOI: 10.1016/0730-725x(82)90172-2. View

Sepponen R, Pohjonen J, Sipponen J, Tanttu J . A method for T1 rho imaging. J Comput Assist Tomogr. 1985; 9(6):1007-11. DOI: 10.1097/00004728-198511000-00002. View

Fullerton G, Cameron I, Ord V . Frequency dependence of magnetic resonance spin-lattice relaxation of protons in biological materials. Radiology. 1984; 151(1):135-8. DOI: 10.1148/radiology.151.1.6322223. View

Koenig S, Brown 3rd R . Determinants of proton relaxation rates in tissue. Magn Reson Med. 1984; 1(4):437-49. DOI: 10.1002/mrm.1910010404. View

Brown J, Vansonnenberg E, Gerber K, Strich G, Wittich G, Slutsky R . Magnetic resonance relaxation times of percutaneously obtained normal and abnormal body fluids. Radiology. 1985; 154(3):727-31. DOI: 10.1148/radiology.154.3.3969478. View

Allen P, Castro M, Treiber E, Lunt J, Boisvert D . A proton NMR relaxation evaluation of a model of brain oedema fluid. Phys Med Biol. 1986; 31(7):699-711. DOI: 10.1088/0031-9155/31/7/001. View

10.

Diegel J, Pintar M . Origin of the nonexponentiality of the water proton spin relaxations in tissues. Biophys J. 1975; 15(9):855-60. PMC: 1334747. DOI: 10.1016/S0006-3495(75)85861-9. View

11.

Koenig S, Schillinger W . Nuclear magnetic relaxation dispersion in protein solutions. I. Apotransferrin. J Biol Chem. 1969; 244(12):3283-9. View

12.

Cooke R, Kuntz I . The properties of water in biological systems. Annu Rev Biophys Bioeng. 1974; 3(0):95-126. DOI: 10.1146/annurev.bb.03.060174.000523. View

13.

Belton P, Packer K . Pulsed NMR studies of water in striated muscle. 3. The effects of water content. Biochim Biophys Acta. 1974; 354(2):305-14. DOI: 10.1016/0304-4165(74)90015-4. View

14.

Fung B, McGaughy T . The state of water in muscle as studied by pulsed NMR. Biochim Biophys Acta. 1974; 343(3):663-73. DOI: 10.1016/0304-4165(74)90287-6. View

15.

Hazlewood C, Chang D, Nichols B, Woessner D . Nuclear magnetic resonance transverse relaxation times of water protons in skeletal muscle. Biophys J. 1974; 14(8):583-606. PMC: 1334554. DOI: 10.1016/S0006-3495(74)85937-0. View

16.

Inch W, McCredie J, Geiger C, Boctor Y . Spin-lattice relaxation times for mixtures of water and gelatin or cotton, compared with normal and malignant tissue. J Natl Cancer Inst. 1974; 53(3):689-90. DOI: 10.1093/jnci/53.3.689. View

17.

Clark M, Burnell E, Chapman N, HINKE J . Water in barnacle muscle. IV. Factors contributing to reduced self-diffusion. Biophys J. 1982; 39(3):289-99. PMC: 1328946. DOI: 10.1016/S0006-3495(82)84519-0. View

18.

Koenig S, Bryant R, Hallenga K, Jacob G . Magnetic cross-relaxation among protons in protein solutions. Biochemistry. 1978; 17(20):4348-58. DOI: 10.1021/bi00613a037. View

19.

Grosch L, Noack F . NMR relaxation investigation of water mobility in aqueous bovine serum albumin solutions. Biochim Biophys Acta. 1976; 453(1):218-32. DOI: 10.1016/0005-2795(76)90267-1. View

20.

. The NMR studies of water in biological systems. Prog Biophys Mol Biol. 1979; 35(2):103-34. DOI: 10.1016/0079-6107(80)90004-8. View