Nucleolus Organizer Regions in Human Lymphocytes As Studied with Premature Chromosome Condensation

Overview

Journal Hum Genet

Specialty Genetics

Date 1990 Feb 1

PMID 1689268

Citations 6

Authors

F Wachtler

C Roubicek

A Schedle

W Mosgoller

G Bretis

H G SCHWARZACHER

Affiliations

Soon will be listed here.

Abstract

Non-stimulated human lymphocytes from peripheral blood usually contain only one ring-shaped nucleolus. Polyethyleneglycol-mediated cell fusion with mitotic Chinese hamster ovary cells induces premature chromosome condensation in human lymphocytes. Subsequent silver staining reveals that more than one nucleolus organizer region (NOR) is silver-positive and frequently participates in the formation of "satellite associations". It can therefore be concluded that more than one NOR contributes to the ring-shaped nucleoli of lymphocytes in human peripheral blood and that they may be transcriptionally active. During phytohemagglutinin (PHA) stimulation, the number of silver-positive NORs, the number of nucleoli and the number of chromosomes participating in "satellite associations" increase.

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