NKT Cells in the Induced Sputum of Severe Asthmatics

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2006 Aug 3

PMID 16883065

Citations 19

Authors

Agnes Hamzaoui

Sana Cheik Rouhou

Hedia Grairi

Hanadi Abid

Jamel Ammar

Hanene Chelbi

Kamel Hamzaoui

Affiliations

Soon will be listed here.

Abstract

To determine whether there was a specific inflammatory process in severe asthmatics, the phenotypic characteristics of induced sputum immune cells were analysed among patients with severe asthma. Twenty-two induced sputa (10 severe asthmatics) were studied. Flow cytometric analysis was performed using immune cells of the sputum and monoclonal antibodies to CD3, CD4, CD8, CD56, CD25, and TCRgammadelta. The number of NKT (CD3(+) CD56(+)) cells was significantly higher in the sputum of severe asthmatics compared with mild asthmatic and healthy control groups (P < .05). CD8(+)CD56(+) cells were the predominant subtype of the increased NKT cells in severe asthmatics. CD3(+)CD56(+)Valpha24(+), TCRgammadelta(+) CD56(+), and CD4(+)CD25(+) T cells were significantly increased in severe asthmatic patients. These results suggest that the immunopathogenesis of severe asthmatics vary between severe and mild asthmatics, and that CD8(+)CD56(+) NKT cells may play an important role in the immunopathogenesis of severe asthma.

Citing Articles

Natural killer T cells in allergic asthma: implications for the development of novel immunotherapeutical strategies.

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De novo fatty-acid synthesis protects invariant NKT cells from cell death, thereby promoting their homeostasis and pathogenic roles in airway hyperresponsiveness.

Koh J, Woo Y, Yoo H, Choi J, Kim S, Chang Y Elife. 2023; 12.

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Licari A, Manti S, Castagnoli R, Leonardi S, Marseglia G Breathe (Sheff). 2020; 16(1):190301.

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Role of CD1d- and MR1-Restricted T Cells in Asthma.

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Development of Asthma in Inner-City Children: Possible Roles of MAIT Cells and Variation in the Home Environment.

Chandra S, Wingender G, Greenbaum J, Khurana A, Gholami A, Ganesan A J Immunol. 2018; 200(6):1995-2003.

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References

Jatakanon A, Uasuf C, Maziak W, Lim S, Chung K, Barnes P . Neutrophilic inflammation in severe persistent asthma. Am J Respir Crit Care Med. 1999; 160(5 Pt 1):1532-9. DOI: 10.1164/ajrccm.160.5.9806170. View

Hamzaoui A, Chaouch N, Grairi H, Ammar J, Hamzaoui K . Inflammatory process of CD8+ CD28- T cells in induced sputum from asthmatic patients. Mediators Inflamm. 2005; 2005(3):160-6. PMC: 1526471. DOI: 10.1155/MI.2005.160. View

Hayday A . [gamma][delta] cells: a right time and a right place for a conserved third way of protection. Annu Rev Immunol. 2000; 18:975-1026. DOI: 10.1146/annurev.immunol.18.1.975. View

Muro S, Minshall E, Hamid Q . The pathology of chronic asthma. Clin Chest Med. 2000; 21(2):225-44. DOI: 10.1016/s0272-5231(05)70263-x. View

Colavita A, Reinach A, Peters S . Contributing factors to the pathobiology of asthma. The Th1/Th2 paradigm. Clin Chest Med. 2000; 21(2):263-77, viii. DOI: 10.1016/s0272-5231(05)70265-3. View

Druilhe A, Letuve S, Pretolani M . [Eosinophil apoptosis in asthma]. Pathol Biol (Paris). 2000; 48(6):566-73. View

Godfrey D, Hammond K, Poulton L, Smyth M, Baxter A . NKT cells: facts, functions and fallacies. Immunol Today. 2000; 21(11):573-83. DOI: 10.1016/s0167-5699(00)01735-7. View

Smyth M, Godfrey D . NKT cells and tumor immunity--a double-edged sword. Nat Immunol. 2001; 1(6):459-60. DOI: 10.1038/82698. View

Shi F, Ljunggren H, Sarvetnick N . Innate immunity and autoimmunity: from self-protection to self-destruction. Trends Immunol. 2001; 22(2):97-101. DOI: 10.1016/s1471-4906(00)01821-4. View

10.

Gibson P, Simpson J, Saltos N . Heterogeneity of airway inflammation in persistent asthma : evidence of neutrophilic inflammation and increased sputum interleukin-8. Chest. 2001; 119(5):1329-36. DOI: 10.1378/chest.119.5.1329. View

11.

Ohkawa T, Seki S, Dobashi H, Koike Y, Habu Y, Ami K . Systematic characterization of human CD8+ T cells with natural killer cell markers in comparison with natural killer cells and normal CD8+ T cells. Immunology. 2001; 103(3):281-90. PMC: 1783250. DOI: 10.1046/j.1365-2567.2001.01248.x. View

12.

van der Vliet H, von Blomberg B, Nishi N, Reijm M, Voskuyl A, van Bodegraven A . Circulating V(alpha24+) Vbeta11+ NKT cell numbers are decreased in a wide variety of diseases that are characterized by autoreactive tissue damage. Clin Immunol. 2001; 100(2):144-8. DOI: 10.1006/clim.2001.5060. View

13.

Komai-Koma M, McKay A, Thomson L, McSharry C, Chalmers G, Liew F . Immuno-regulatory cytokines in asthma: IL-15 and IL-13 in induced sputum. Clin Exp Allergy. 2001; 31(9):1441-8. DOI: 10.1046/j.1365-2222.2001.01174.x. View

14.

Mempel M, Ronet C, Suarez F, Gilleron M, Puzo G, Van Kaer L . Natural killer T cells restricted by the monomorphic MHC class 1b CD1d1 molecules behave like inflammatory cells. J Immunol. 2001; 168(1):365-71. DOI: 10.4049/jimmunol.168.1.365. View

15.

Hamzaoui A, Kahan A, Ayed K, Hamzaoui K . T cells expressing the gammadelta receptor are essential for Th2-mediated inflammation in patients with acute exacerbation of asthma. Mediators Inflamm. 2002; 11(2):113-9. PMC: 1781649. DOI: 10.1080/09629350220131971. View

16.

Kukreja A, Costi G, Marker J, Zhang C, Sinha S, Sun Z . NKT cell defects in NOD mice suggest therapeutic opportunities. J Autoimmun. 2002; 19(3):117-28. DOI: 10.1006/jaut.2002.0609. View

17.

Brightling C, Symon F, Birring S, Bradding P, Pavord I, Wardlaw A . TH2 cytokine expression in bronchoalveolar lavage fluid T lymphocytes and bronchial submucosa is a feature of asthma and eosinophilic bronchitis. J Allergy Clin Immunol. 2002; 110(6):899-905. DOI: 10.1067/mai.2002.129698. View

18.

Leckie M, Jenkins G, Khan J, Smith S, Walker C, Barnes P . Sputum T lymphocytes in asthma, COPD and healthy subjects have the phenotype of activated intraepithelial T cells (CD69+ CD103+). Thorax. 2003; 58(1):23-9. PMC: 1746466. DOI: 10.1136/thorax.58.1.23. View

19.

Fabbri L, Romagnoli M, Corbetta L, Casoni G, Busljetic K, Turato G . Differences in airway inflammation in patients with fixed airflow obstruction due to asthma or chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 2002; 167(3):418-24. DOI: 10.1164/rccm.200203-183OC. View

20.

Tsoumakidou M, Tzanakis N, Kyriakou D, Chrysofakis G, Siafakas N . Inflammatory cell profiles and T-lymphocyte subsets in chronic obstructive pulmonary disease and severe persistent asthma. Clin Exp Allergy. 2004; 34(2):234-40. DOI: 10.1111/j.1365-2222.2004.01858.x. View