Model for Human Skin Reconstructed in Vitro Composed of Associated Dermis and Epidermis

Overview

Journal Sao Paulo Med J

Specialty General Medicine

Date 2006 Aug 1

PMID 16878189

Citations 12

Authors

Luis Ricardo Martinhao Souto

Jussara Rehder

Jose Vassallo

Maria Leticia Cintra

Maria Helena Stangler Kraemer

Maria Beatriz Puzzi

Affiliations

Soon will be listed here.

Abstract

Context And Objective: The technique of obtaining human skin with dermis and epidermis reconstructed from cells isolated from patients can enable autologous skin grafting on patients with few donor sites. It also enables in vitro trials on chemicals and drugs. The objective of this work was to demonstrate a method for obtaining human skin composed of associated dermis and epidermis, reconstructed in vitro.

Design And Setting: Experimental laboratory study, in the Skin Cell Culture Laboratory of Faculdade de Ciências Médicas, Universidade Estadual de Campinas.

Methods: Cells from human fibroblast cultures are injected into bovine collagen type I matrix and kept immersed in specific culturing medium for fibroblasts. This enables human dermis reconstruction in vitro. On this, by culturing human keratinocytes and melanocytes, differentiated epidermis is formed, leading to the creation of human skin composed of associated dermis and epidermis, reconstructed in vitro.

Results: We showed that human skin composed of associated dermis and epidermis can be successfully reconstructed in vitro. It is histologically formed in the same way as human skin in vivo. Collagen tissue can be identified in the dermis, with cells and extracellular matrix organized in parallel to multilayer epidermis.

Conclusions: It is possible to obtain completely differentiated human skin composed of associated dermis and epidermis, reconstructed in vitro, from injection of human fibroblasts into bovine collagen type I matrix and culturing of human keratinocytes and melanocytes on this matrix.

Citing Articles

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A novel optical coherence tomography-based in vitro method of anti-aging skin analysis using 3D skin wrinkle mimics.

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The effect of aging in primary human dermal fibroblasts.

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Current and Future Perspectives on Skin Tissue Engineering: Key Features of Biomedical Research, Translational Assessment, and Clinical Application.

Yu J, Navarro J, Coburn J, Mahadik B, Molnar J, Holmes 4th J Adv Healthc Mater. 2019; 8(5):e1801471.

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Modeling Barrier Tissues In Vitro: Methods, Achievements, and Challenges.

Sakolish C, Esch M, Hickman J, Shuler M, Mahler G EBioMedicine. 2016; 5:30-9.

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