Intraspecific DNA Content Variability in Festuca Pallens on Different Geographical Scales and Ploidy Levels

Overview

Journal Ann Bot

Publisher Oxford University Press

Specialty Biology

Date 2006 Jul 27

PMID 16868002

Citations 25

Authors

Petr Smarda

Petr Bures

Affiliations

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Abstract

Background And Aims: Intraspecific genome size variability of Festuca pallens occurring on relict rocky steppes in Central Europe was studied on two ploidy levels and three geographical scales: (1) local scale of 24 populations, (2) landscape scale of three transects in river canyons or hill systems, and (3) global scale of 160 samples covering the whole distribution area.

Methods: DAPI flow cytometry of homogeneously cultivated samples (>or=1 year), measured randomly with two internal standards, Lycopersicon esculentum and Pisum sativum. Differences in DNA content were confirmed (1) by the double peaks of simultaneously measured samples, (2) based on measurements carried out in different seasons, and (3) by additional measurements with propidium iodide.

Key Results: On a global scale, the relative DNA content ranged between 1.170-fold in diploids and 1.164-fold in tetraploids. A maximum difference of 1.088-fold between the mean relative DNA content of nearby populations was found. In 16 of 24 populations significant variability was shown (P<0.001, 1.121-fold as maximum). For both ploidy levels, the relative genome size had the same range and geographical pattern, correlated with geographical coordinates (P<0.01). Diploids with larger genomes occur on relict habitats (P<0.01), and in areas of periglacial steppes (20,000 years ago; P<0.02). In tetraploids, the relative DNA content differs among the three previously recognized geographical types (Alpine, Pannonian and Scabrifolia, P<0.001). Tetraploids have a relative DNA content smaller than twice that of the diploids (P<0.001). An influence of microhabitat on DNA content variation was not confirmed.

Conclusions: Genome size variability occurs over all spatial scales: intrapopulation, landscape and global. Correlation between geographical coordinates and palaeovegetation type, concomitant with diploids and tetraploids, and no influence of microhabitat were found. Genome size decreases in tetraploids. Lower CVs, and thus higher accuracy, resolution and reproducibility, favour DAPI measurements for the study of intraspecific genome size variability.

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References

Johnston J, Bennett M, Rayburn A, Galbraith D, Price H . Reference standards for determination of DNA content of plant nuclei. Am J Bot. 1999; 86(5):609-13. View

Rai K, Black 4th W . Mosquito genomes: structure, organization, and evolution. Adv Genet. 1999; 41:1-33. DOI: 10.1016/s0065-2660(08)60149-2. View

Turpeinen , Kulmala , Nevo . Genome size variation in Hordeum spontaneum populations. Genome. 2000; 42(6):1094-9. DOI: 10.1139/g99-066. View

Kalendar R, Tanskanen J, Immonen S, Nevo E, Schulman A . Genome evolution of wild barley (Hordeum spontaneum) by BARE-1 retrotransposon dynamics in response to sharp microclimatic divergence. Proc Natl Acad Sci U S A. 2000; 97(12):6603-7. PMC: 18673. DOI: 10.1073/pnas.110587497. View

Temsch E, Greilhuber J . Genome size variation in Arachis hypogaea and A. monticola re-evaluated. Genome. 2000; 43(3):449-51. View

Nevo E . Evolution of genome-phenome diversity under environmental stress. Proc Natl Acad Sci U S A. 2001; 98(11):6233-40. PMC: 33451. DOI: 10.1073/pnas.101109298. View

Barow M, Meister A . Lack of correlation between AT frequency and genome size in higher plants and the effect of nonrandomness of base sequences on dye binding. Cytometry. 2002; 47(1):1-7. DOI: 10.1002/cyto.10030. View

Noirot M, Barre P, Duperray C, Louarn J, Hamon S . Effects of caffeine and chlorogenic acid on propidium iodide accessibility to DNA: consequences on genome size evaluation in coffee tree. Ann Bot. 2003; 92(2):259-64. PMC: 4243661. DOI: 10.1093/aob/mcg139. View

Jones N, Houben A . B chromosomes in plants: escapees from the A chromosome genome?. Trends Plant Sci. 2003; 8(9):417-23. DOI: 10.1016/S1360-1385(03)00187-0. View

10.

Jakob S, Meister A, Blattner F . The considerable genome size variation of Hordeum species (poaceae) is linked to phylogeny, life form, ecology, and speciation rates. Mol Biol Evol. 2004; 21(5):860-9. DOI: 10.1093/molbev/msh092. View

11.

Bures P, Pavlicek T, Horova L, Nevo E . Microgeographic genome size differentiation of the carob tree, Ceratonia siliqua, at 'Evolution Canyon', Israel. Ann Bot. 2004; 93(5):529-35. PMC: 4242316. DOI: 10.1093/aob/mch074. View

12.

Bures P, Wang Y, Horova L, Suda J . Genome size variation in Central European species of Cirsium (Compositae) and their natural hybrids. Ann Bot. 2004; 94(3):353-63. PMC: 4242176. DOI: 10.1093/aob/mch151. View

13.

Jones R, Diez M . The B chromosome database. Cytogenet Genome Res. 2004; 106(2-4):149-50. DOI: 10.1159/000079280. View

14.

Delgado M, Caperta A, Ribeiro T, Viegas W, Jones R, Morais-Cecilio L . Different numbers of rye B chromosomes induce identical compaction changes in distinct A chromosome domains. Cytogenet Genome Res. 2004; 106(2-4):320-4. DOI: 10.1159/000079306. View

15.

Nokkala S, Nokkala C . Interaction of B chromosomes with A or B chromosomes in segregation in insects. Cytogenet Genome Res. 2004; 106(2-4):394-7. DOI: 10.1159/000079317. View

16.

Greilhuber J . Intraspecific variation in genome size in angiosperms: identifying its existence. Ann Bot. 2004; 95(1):91-8. PMC: 4246709. DOI: 10.1093/aob/mci004. View

17.

Dolezel J, Bartos J . Plant DNA flow cytometry and estimation of nuclear genome size. Ann Bot. 2004; 95(1):99-110. PMC: 4246710. DOI: 10.1093/aob/mci005. View

18.

Noirot M, Barre P, Duperray C, Hamon S, de Kochko A . Investigation on the causes of stoichiometric error in genome size estimation using heat experiments: consequences on data interpretation. Ann Bot. 2004; 95(1):111-8. PMC: 4246711. DOI: 10.1093/aob/mci006. View

19.

Murray B . When does intraspecific C-value variation become taxonomically significant?. Ann Bot. 2004; 95(1):119-25. PMC: 4246712. DOI: 10.1093/aob/mci007. View

20.

Bennetzen J, Ma J, Devos K . Mechanisms of recent genome size variation in flowering plants. Ann Bot. 2004; 95(1):127-32. PMC: 4246713. DOI: 10.1093/aob/mci008. View