Effect of Ferric Nitrilotriacetate on Rostral Mesencephalic Cells

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1991 Dec 1

PMID 1686065

Citations 1

Authors

K F SWAIMAN

V L Machen

Affiliations

Soon will be listed here.

Abstract

After murine fetal cells from the rostral mesencephalic tegmentum were isolated, prepared, and cultured; neuronal and glial cells in primary mixed cell cultures were exposed to ferric nitrilotriacetate (Fe-NTA) at varying concentrations. Studies were performed at 23 days in culture after 14 day exposure to Fe-NTA. In addition to morphologic studies, biochemical assays including specific [3H]flunitrazepam (FLU) binding, clonazepam (CLO)-displaceable [3H]-FLU binding, Ro5-4864-displaceable [3H]-FLU binding, [3H]-FLU binding, [3H]dopamine (DA) uptake, [3H]haloperidol (HAL) binding, [3H]spiperone (SP) binding, glutamine synthetase activity (GS), and protein determinations were performed. The data demonstrate that chelated ferric iron has an adverse effect on these cells. The data also demonstrate that increasing concentrations of Fe-NTA resulted in massive neuronal dropout leaving the culture population virtually all glial; however, the specific binding of [3H]HAL and [3H]SP increased. There was a concomitant decrease in both glutamine synthetase activity and overall protein content. The mechanism of enhancement in the presence of Fe-NTA of [3H]HAL and [3H]SP binding is unknown and may be unique, but may be related to the known increase in D2 receptor ligand affinity in the presence of other multivalent cations (Ca2+ and Mg2+).

Citing Articles

Changes in iron-regulatory proteins in the aged rodent neural retina.

Chen H, Liu B, Lukas T, Suyeoka G, Wu G, Neufeld A Neurobiol Aging. 2008; 30(11):1865-76.

PMID: 18308429 PMC: 2789556. DOI: 10.1016/j.neurobiolaging.2008.01.002.

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