» Articles » PMID: 16828752

Involvement of Rho/ROCK Signalling in Small Cell Lung Cancer Migration Through Human Brain Microvascular Endothelial Cells

Overview
Journal FEBS Lett
Specialty Biochemistry
Date 2006 Jul 11
PMID 16828752
Citations 69
Authors
Affiliations
Soon will be listed here.
Abstract

Small cell lung cancer (SCLC) cells migration across human brain microvascular endothelial cells (HBMECs) is an essential step of brain metastases. Here we investigated signalling pathways in HBMECs contributing to the process. Inhibition of endothelial Rho kinase (ROCK) with Y27632 and overexpression of ROCK dominant-negative mutant prevented SCLC cells, NCI-H209, transendothelial migration and the concomitant changes of tight junction. Conversely, inhibition of phosphatidylinositol 3-kinase (PI3K) and protein kinase C (PKC) had no effects. Furthermore, endothelial RhoA protein was activated during NCI-H209 cells transendothelial migration. Rho/ROCK participated in NCI-H209 cells transendothelial migration through regulating actin cytoskeleton reorganization. These results suggested that Rho/ROCK was required for SCLC cells transendothelial migration.

Citing Articles

The T-type voltage-gated Ca channel Ca3.1 involves in the disruption of respiratory epithelial barrier induced by toxin.

Bi H, Wang F, Lin L, Zhang D, Chen M, Shang Y Virulence. 2025; 16(1):2466482.

PMID: 39950866 PMC: 11834503. DOI: 10.1080/21505594.2025.2466482.


Breaking boundaries: role of the brain barriers in metastatic process.

Izadi N, Solar P, Hasanova K, Zamani A, Akbar M, Mrazova K Fluids Barriers CNS. 2025; 22(1):3.

PMID: 39780275 PMC: 11708195. DOI: 10.1186/s12987-025-00618-z.


Junctions at the crossroads: the impact of mechanical cues on endothelial cell-cell junction conformations and vascular permeability.

Brandon K, Frank W, Stroka K Am J Physiol Cell Physiol. 2024; 327(4):C1073-C1086.

PMID: 39129490 PMC: 11481987. DOI: 10.1152/ajpcell.00605.2023.


Breaking barriers: exploring mechanisms behind opening the blood-brain barrier.

Stamp M, Halwes M, Nisbet D, Collins D Fluids Barriers CNS. 2023; 20(1):87.

PMID: 38017530 PMC: 10683235. DOI: 10.1186/s12987-023-00489-2.


Insights into the Molecular Mechanisms Mediating Extravasation in Brain Metastasis of Breast Cancer, Melanoma, and Lung Cancer.

Alsabbagh R, Ahmed M, Alqudah M, Hamoudi R, Harati R Cancers (Basel). 2023; 15(8).

PMID: 37190188 PMC: 10137309. DOI: 10.3390/cancers15082258.