Phase-variable Surface Structures Are Required for Infection of Campylobacter Jejuni by Bacteriophages

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2006 Jul 6

PMID 16820455

Citations 40

Authors

Chris Coward

Andrew J Grant

Craig Swift

Jennifer Philp

Rebecca Towler

Mohammad Heydarian

Jennifer A Frost

Duncan J Maskell

Affiliations

Soon will be listed here.

Abstract

This study characterizes the interaction between Campylobacter jejuni and the 16 phages used in the United Kingdom typing scheme by screening spontaneous mutants of the phage-type strains and transposon mutants of the sequenced strain NCTC 11168. We show that the 16 typing phages fall into four groups based on their patterns of activity against spontaneous mutants. Screens of transposon and defined mutants indicate that the phage-bacterium interaction for one of these groups appears to involve the capsular polysaccharide (CPS), while two of the other three groups consist of flagellatropic phages. The expression of CPS and flagella is potentially phase variable in C. jejuni, and the implications of these findings for typing and intervention strategies are discussed.

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