Cloning and Characterization of a Root-specific Expressing Gene Encoding 3-hydroxy-3-methylglutaryl Coenzyme A Reductase from Ginkgo Biloba

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2006 Jul 4

PMID 16817021

Citations 25

Authors

Guoan Shen

Yongzhen Pang

Weisheng Wu

Zhihua Liao

Lingxia Zhao

Xiaofen Sun

Kexuan Tang

Affiliations

Soon will be listed here.

Abstract

3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR, EC: 1.1.1.34) catalyzes the first committed step in mevalonic acid (MVA) pathway for biosynthesis of isoprenoids. The full-length cDNA encoding HMGR was isolated from Ginkgo biloba for the first time (designated as GbHMGR, GenBank accession number AY741133), which contained a 1713 bp ORF encoding 571 amino acids. The GbHMGR genomic DNA sequence was also obtained, revealing GbHMGR had four exons and three introns. The deduced GbHMGR protein showed high identity to other plant HMGRs and contained two trans-membrane domains and a catalytic domain. The three dimensional model of GbHMGR represented a typical spatial structure of HMGRs. The Southern blot and RT-PCR assay results indicated that GbHMGR belonged to a small gene family, and expressed in a tissue-specific manner with a low level expression being only found in root. The potential significance of GbHMGR gene was also discussed.

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