Pattern of MRNA Expression of Beta-defensins in Basal Cell Carcinoma

Overview

Journal BMC Cancer

Publisher Biomed Central

Specialty Oncology

Date 2006 Jun 27

PMID 16796735

Citations 29

Authors

T Gambichler

M Skrygan

J Huyn

F G Bechara

M Sand

P Altmeyer

A Kreuter

Affiliations

Soon will be listed here.

Abstract

Background: Although the human beta-defensins hBDs today seem to have diverse functional activities in innate antimicrobial immunity, a few reports also indicated an altered expression of these antimicrobial peptides (AMPs) in tissues of cancers such as oral squamous cell carcinoma. The present work was aimed on the study of hBD gene expression in basal cell carcinoma (BCC) which is the most common cancer in humans.

Methods: Twenty-two non-ulcerated BCCs (12 nodular type, 10 superficial type) have been analysed for the presence of hBD (1-3) mRNA by quantitative real-time RT-PCR. As controls, non-lesional skin specimens of BCC patients as well as samples of healthy subjects were assessed by RT-PCR.

Results: hBD-1 levels in healthy controls and non-lesional skin of BCC patients were significantly (P < 0.05) higher than the levels observed in tumour tissue. Moreover, BCCs showed significantly (P < 0.05) increased mRNA expression of hBD-2 as compared to controls. There was no significant (P > 0.05) difference between lesional mRNA levels for hBD-3 and those levels observed in controls. The mRNA expression of hBDs (1-3) found in nodular and superficial BCCs did not significantly (P > 0.05) differ.

Conclusion: The gene expression patterns of hBD-1 and hBD-2 are for the first time shown to be significantly altered in non-ulcerated BCCs as compared to intra-individual and inter-individual controls, respectively. The present findings may indicate that beside the antimicrobial activity of AMPs, hBDs may also play a role in the pathogenesis of BCC. However, functional and immunohistological studies investigating hBDs in patients with BCC are needed to confirm our data.

Citing Articles

Defensins: Exploring Their Opposing Roles in Colorectal Cancer Progression.

Sabit H, Pawlik T, Abdel-Ghany S, Arneth B Cancers (Basel). 2024; 16(15).

PMID: 39123348 PMC: 11311076. DOI: 10.3390/cancers16152622.

Microbiota in cancer: molecular mechanisms and therapeutic interventions.

Chen Z, Guan D, Wang Z, Li X, Dong S, Huang J MedComm (2020). 2023; 4(6):e417.

PMID: 37937304 PMC: 10626288. DOI: 10.1002/mco2.417.

Mechanisms and regulation of defensins in host defense.

Fu J, Zong X, Jin M, Min J, Wang F, Wang Y Signal Transduct Target Ther. 2023; 8(1):300.

PMID: 37574471 PMC: 10423725. DOI: 10.1038/s41392-023-01553-x.

The role of TGF-β and antimicrobial peptides in basal cell carcinoma: a systematic review.

Fijalkowska M, Bonczar M, Jastrzebski I, Ostrowski P, Antoszewski B, Koziej M Postepy Dermatol Alergol. 2023; 40(3):384-389.

PMID: 37545828 PMC: 10399676. DOI: 10.5114/ada.2023.124747.

Serum β-Defensin 2, A Novel Biomarker for the Diagnosis of Acute Infections.

Routsias J, Marinou D, Mavrouli M, Tsakris A, Pitiriga V Diagnostics (Basel). 2023; 13(11).

PMID: 37296737 PMC: 10252252. DOI: 10.3390/diagnostics13111885.

References

Mizukawa N, Sugiyama K, Kamio M, Yamachika E, Ueno T, Fukunaga J . Immunohistochemical staining of human alpha-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas. Anticancer Res. 2000; 20(2B):1125-7. View

Gambichler T, Skrygan M, Tomi N, Altmeyer P, Kreuter A . Changes of antimicrobial peptide mRNA expression in atopic eczema following phototherapy. Br J Dermatol. 2006; 155(6):1275-8. DOI: 10.1111/j.1365-2133.2006.07481.x. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Muller C, Markovic-Lipkovski J, Klatt T, Gamper J, Schwarz G, Beck H . Human alpha-defensins HNPs-1, -2, and -3 in renal cell carcinoma: influences on tumor cell proliferation. Am J Pathol. 2002; 160(4):1311-24. PMC: 1867209. DOI: 10.1016/s0002-9440(10)62558-8. View

Sawaki K, Yamaai T, Mizukawa N, Yoshimoto T, Ueno T, Nakano M . Mortality of human epidermal keratinocytes in co-culture with oral squamous cell carcinoma cells. Anticancer Res. 2003; 23(1A):79-84. View

Donald C, Sun C, Lim S, Macoska J, Cohen C, Amin M . Cancer-specific loss of beta-defensin 1 in renal and prostatic carcinomas. Lab Invest. 2003; 83(4):501-5. DOI: 10.1097/01.lab.0000063929.61760.f6. View

Iftner A, Klug S, Garbe C, Blum A, Stancu A, Wilczynski S . The prevalence of human papillomavirus genotypes in nonmelanoma skin cancers of nonimmunosuppressed individuals identifies high-risk genital types as possible risk factors. Cancer Res. 2003; 63(21):7515-9. View

Yoshimoto T, Yamaai T, Mizukawa N, Sawaki K, Nakano M, Yamachika E . Different expression patterns of beta-defensins in human squamous cell carcinomas. Anticancer Res. 2004; 23(6C):4629-33. View

Jappe U, Petzoldt D, Wendt C . Methicillin-resistant Staphylococcus aureus colonization in inflammatory versus non-inflammatory skin diseases: who should be screened?. Acta Derm Venereol. 2004; 84(3):181-6. DOI: 10.1080/00015550410025255. View

10.

Zucht H, Grabowsky J, Schrader M, Liepke C, Jurgens M, Schulz-Knappe P . Human beta-defensin-1: A urinary peptide present in variant molecular forms and its putative functional implication. Eur J Med Res. 1998; 3(7):315-23. View

11.

Abiko Y, Mitamura J, Nishimura M, Muramatsu T, Inoue T, Shimono M . Pattern of expression of beta-defensins in oral squamous cell carcinoma. Cancer Lett. 1999; 143(1):37-43. DOI: 10.1016/s0304-3835(99)00171-8. View

12.

Yang D, Chertov O, Bykovskaia S, Chen Q, Buffo M, Shogan J . Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6. Science. 1999; 286(5439):525-8. DOI: 10.1126/science.286.5439.525. View

13.

Shnitsar V, Lisovskiy I, Soldatkina M, Nespryadko S, Turchak O, Vinnitskaya A . Human beta-defensin 3 (hBD-3) expression in A431 cell line and human vulval tumors. Exp Oncol. 2005; 26(4):328-30. View

14.

Albrethsen J, Bogebo R, Gammeltoft S, Olsen J, Winther B, Raskov H . Upregulated expression of human neutrophil peptides 1, 2 and 3 (HNP 1-3) in colon cancer serum and tumours: a biomarker study. BMC Cancer. 2005; 5:8. PMC: 548152. DOI: 10.1186/1471-2407-5-8. View

15.

Arimura Y, Ashitani J, Yanagi S, Tokojima M, Abe K, Mukae H . Elevated serum beta-defensins concentrations in patients with lung cancer. Anticancer Res. 2005; 24(6):4051-7. View

16.

Izadpanah A, Gallo R . Antimicrobial peptides. J Am Acad Dermatol. 2005; 52(3 Pt 1):381-90. DOI: 10.1016/j.jaad.2004.08.026. View

17.

Daya-Grosjean L, Couve-Privat S . Sonic hedgehog signaling in basal cell carcinomas. Cancer Lett. 2005; 225(2):181-92. DOI: 10.1016/j.canlet.2004.10.003. View

18.

Kuzmina N, Talme T, Lapins J, Emtestam L . Non-invasive preoperative assessment of basal cell carcinoma of nodular and superficial types. Skin Res Technol. 2005; 11(3):196-200. DOI: 10.1111/j.1600-0846.2005.00120.x. View

19.

Rubin A, Chen E, Ratner D . Basal-cell carcinoma. N Engl J Med. 2005; 353(21):2262-9. DOI: 10.1056/NEJMra044151. View

20.

de Jongh G, Zeeuwen P, Kucharekova M, Pfundt R, Van der Valk P, Blokx W . High expression levels of keratinocyte antimicrobial proteins in psoriasis compared with atopic dermatitis. J Invest Dermatol. 2005; 125(6):1163-73. DOI: 10.1111/j.0022-202X.2005.23935.x. View