Multidimensional Single-molecule Imaging in Live Cells Using Total-internal-reflection Fluorescence Microscopy

Overview

Journal Opt Lett

Specialty Ophthalmology

Date 2006 Jun 24

PMID 16794711

Citations 14

Authors

S E D Webb

S R Needham

S K Roberts

M L Martin-Fernandez

Affiliations

Soon will be listed here.

Abstract

We have developed a wide-field total-internal-reflection fluorescence microscope capable of imaging single molecules in live cells, resolved in both wavelength and polarization. We show fluorescence resonance energy transfer between single pairs of fluorescent molecules bound to signaling receptors in the plasma membrane of live cells and demonstrate the importance of polarization discrimination in addition to wavelength separation.

Citing Articles

A perspective of fluorescence microscopy for cellular structural biology with EGFR as witness.

Martin-Fernandez M J Microsc. 2022; 291(1):73-91.

PMID: 36282005 PMC: 10952613. DOI: 10.1111/jmi.13151.

Fluorescence Imaging of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Resistance in Non-Small Cell Lung Cancer.

Martin-Fernandez M Cancers (Basel). 2022; 14(3).

PMID: 35158954 PMC: 8833717. DOI: 10.3390/cancers14030686.

A small molecule inhibitor of HER3: a proof-of-concept study.

Colomba A, Fitzek M, George R, Weitsman G, Roberts S, Zanetti-Domingues L Biochem J. 2020; 477(17):3329-3347.

PMID: 32815546 PMC: 7489893. DOI: 10.1042/BCJ20200496.

A Brief History of Single-Particle Tracking of the Epidermal Growth Factor Receptor.

Clarke D, Martin-Fernandez M Methods Protoc. 2019; 2(1).

PMID: 31164594 PMC: 6481046. DOI: 10.3390/mps2010012.

The architecture of EGFR's basal complexes reveals autoinhibition mechanisms in dimers and oligomers.

Zanetti-Domingues L, Korovesis D, Needham S, Tynan C, Sagawa S, Roberts S Nat Commun. 2018; 9(1):4325.

PMID: 30337523 PMC: 6193980. DOI: 10.1038/s41467-018-06632-0.