Reduced CD4 T Cell Activation and in Vitro Susceptibility to HIV-1 Infection in Exposed Uninfected Central Africans

Overview

Journal Retrovirology

Publisher Biomed Central

Specialty Microbiology

Date 2006 Jun 24

PMID 16792805

Citations 64

Authors

Evelyne Begaud

Loic Chartier

Valery Marechal

Julienne Ipero

Josianne Leal

Pierre Versmisse

Guillaume Breton

Arnaud Fontanet

Corinne Capoulade-Metay

Herve Fleury

Francoise Barre-Sinoussi

Daniel Scott-Algara

Gianfranco Pancino

Affiliations

Soon will be listed here.

Abstract

Background: Environmentally driven immune activation was suggested to contribute to high rates of HIV-1 infection in Africa. We report here a study of immune activation markers and susceptibility to HIV-1 infection in vitro of forty-five highly exposed uninfected partners (EUs) of HIV-1 infected individuals in Central African Republic, in comparison with forty-four low-risk blood donors (UCs).

Results: Analysis of T lymphocyte subsets and activation markers in whole blood showed that the absolute values and the percentage of HLA-DR+CD4 T cells and of CCR5+CD4 T cells were lower in the EUs than in the UCs (p = 0.0001). Mutations in the CCR5 coding region were not found in either group. Susceptibility to in vitro infection of unstimulated peripheral blood mononuclear cells, prior of PHA activation, was decreased in EUs compared to UCs, either using a CXCR4-tropic or a CCR5-tropic HIV-1 strain (p = 0.02 and p = 0.05, respectively). Levels of MIP-1beta, but not of MIP-1alpha or RANTES, in the supernatants of PHA-activated PBMC, were higher in the EUs than in the UCs (p = 0.007).

Conclusion: We found low levels of CD4 T cell activation and reduced PBMC susceptibility to HIV-1 infection in Central African EUs, indicating that both may contribute to the resistance to HIV-1 infection.

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