Localization of Thymosin Beta10 in Breast Cancer Cells: Relationship to Actin Cytoskeletal Remodeling and Cell Motility

Overview

Journal Histochem Cell Biol

Publisher Springer

Specialties Biochemistry
Cell Biology

Date 2006 Jun 21

PMID 16786322

Citations 16

Authors

Aase Elisabeth Maelan

Trine Kring Rasmussen

Lars-Inge Larsson

Affiliations

Soon will be listed here.

Abstract

Beta-thymosins are polypeptides involved in the regulation of actin polymerization and thymosin beta10 and beta4 have been implicated in sequestration of monomeric (G-) actin. Additionally, experimental overexpression of thymosin beta10 has been found to result in increases in F-actin bundles as well as in cell motility and spreading. We have studied the distribution of endogenously expressed thymosin beta10 in cultured human breast cancer cell lines. Both unperturbed monolayer cultures and wound-healing models were examined using double-staining for thymosin beta10 and polymerized (F-) actin. Our findings show that thymosin beta10 is expressed in all three-cancer cell lines (SK-BR-3, MCF-7 and MDA-MB-231) studied. No or little staining was detected in confluent cells, whereas strong staining occurred in semiconfluent cells and in cells populating monolayer wounds. Importantly, the distribution of staining for thymosin beta10 was inverse of staining for F-actin. These data support a physiological role for thymosin beta10 in sequestration of G-actin as well as in cancer cell motility.

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