Ultrastructural Alterations Induced by the Neolignan Dihydrobenzofuranic Eupomatenoid-5 on Epimastigote and Amastigote Forms of Trypanosoma Cruzi

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2006 Jun 20

PMID 16783543

Citations 2

Authors

Patricia Shima Luize

Tania Ueda-Nakamura

Benedito Prado Dias Filho

Diogenes Aparicio Garcia Cortez

Jose Andres Morgado-Diaz

Wanderley De Souza

Celso Vataru Nakamura

Affiliations

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Abstract

Eupomatenoid-5, a compound isolated from leaves of Piper regnellii var. pallescens, showed antiprotozoal activity against the epimastigote proliferative stages and intracellular amastigote forms of Trypanosoma cruzi Y strain. Eupomatenoid-5, at 7.0 microg/ml (50% growth inhibition concentration) produced morphological changes in epimastigote forms of the parasite, such as intense cytoplasmic vacuolization, mitochondrial swelling, kinetoplast alteration, presence of myelin-like figures, and mitochondrial damage, as observed by transmission electron microscopy. In amastigote forms in LLCMK(2) cells, at 7.0-microg/ml concentration, the compound induced a decrease in the number of cells with internalized parasites and in the number of internalized parasites per LLCMK(2) cell, compared with untreated cells. Furthermore, intense cytoplasmic vacuolization and autophagic vacuoles were observed in T. cruzi intracellular amastigotes after 72 h of incubation. Scanning electron microscopy confirmed the alterations in the shape of the parasites.

Citing Articles

Exploring Synthetic Dihydrobenzofuran and Benzofuran Neolignans as Antiprotozoal Agents against .

Pagotti M, Dias H, Candido A, Oliveira T, Borges A, Oliveira N Pharmaceutics. 2023; 15(3).

PMID: 36986617 PMC: 10052957. DOI: 10.3390/pharmaceutics15030754.

Natural compounds based chemotherapeutic against Chagas disease and leishmaniasis: mitochondrion as a strategic target.

Lazarin-Bidoia D, Garcia F, Ueda-Nakamura T, de Oliveira Silva S, Nakamura C Mem Inst Oswaldo Cruz. 2022; 117:e220396.

PMID: 35352776 PMC: 8970591. DOI: 10.1590/0074-02760220396.

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