Relationship Between Enhanced Turnover of Phosphatidylinositol and Lymphocyte Activation by Mitogens

Overview

Journal Biochem J

Specialty Biochemistry

Date 1975 Jan 1

PMID 167716

Citations 33

Authors

V C Maino

M J Hayman

M J Crumpton

Affiliations

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Abstract

1. Various lectins [phaseolus vulgaris phytohaemagglutinin, Glycine max (soy-bean) agglutinin, Triticum vulgaris (wheat-germ) agglutinin and Axinella polyploides agglutinin] and antibodies to pig Ig (immunoglobulin) that are found by pig lymphocytes were assessed in terms of their capacities to stimulate lymphocyte transformation and to enhance phosphatidylinositol turnover. Transformation was measured after 45h of culture by incorporation of [6-(3)H]thymidine into DNA, whereas phosphatidylinositol metabolism was assessed after 1h of cultuis and G. max agglutinins and rabbit antibodies to pig Ig) increased phosphatidylinositol turnover, but non-transforming agents (T. vulgaris and A. polyploides agglutinins and Fab fragments of rabbit antibodies to pig Ig) failed to induce any significant enhancement. Subsequent cross-linkage of the bound, non-transforming Fab fragments with a goat antiserum to rabbit Ig stimulated transformation and phosphatidylinositol turnover. 3. Each transforming agent gave characteristic optimal dose responses that were similar for both phosphatidylinositol turnover and transformation. 4. The results indicate that activation of T- and B-lymphocytes is accompanied by enhanced phosphatidylinositol turnover and that in the case of B-cells this enhancement depends on the cross-linkage of surface receptors. They are consistent with the proposal that turnover represents an essential early step in the transformation process.

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References

Greaves M, Janossy G . Elicitation of selective T and B lymphocyte responses by cell surface binding ligands. Transplant Rev. 1972; 11:87-130. DOI: 10.1111/j.1600-065x.1972.tb00047.x. View

Elson C, Singh J, Taylor R . The effect of capping by anti-immunoglobulin antibody on the expression of cell surface immunoglobulin and on lymphocyte activation. Scand J Immunol. 1973; 2(2):143-9. DOI: 10.1111/j.1365-3083.1973.tb02025.x. View

Stoker M, Macpherson I . SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES. Nature. 1964; 203:1355-7. DOI: 10.1038/2031355a0. View

Allan D, Auger J, Crumpton M . Interaction of phytohemagglutinin with plasma membranes of pig lymphocytes and thymus cells. Exp Cell Res. 1971; 66(2):362-8. DOI: 10.1016/0014-4827(71)90689-6. View

Allen A, Neuberger A, Sharon N . The purification, composition and specificity of wheat-germ agglutinin. Biochem J. 1973; 131(1):155-62. PMC: 1177449. DOI: 10.1042/bj1310155. View

Michell R, Lapetina E . Production of cyclic inositol phosphate in stimulated tissues. Nat New Biol. 1972; 240(104):258-60. DOI: 10.1038/newbio240258a0. View

Allan D, Auger J, Crumpton M . Glycoprotein receptors for concanavalin A isolated from pig lymphocyte plasma membrane by affinity chromatography in sodium deoxycholate. Nat New Biol. 1972; 236(62):23-5. DOI: 10.1038/newbio236023a0. View

Fanger M, Hart D, Wells J, Nisonoff A . Requirement for cross-linkage in the stimulation of transformation of rabbit peripheral lymphocytes by antiglobulin reagents. J Immunol. 1970; 105(6):1484-92. View

Masuzawa Y, Osawa T, Inoue K, Nojima S . Effects of various mitogens on the phospholipid metabolism of human peripheral lymphocytes. Biochim Biophys Acta. 1973; 326(3):339-44. View

10.

HOKIN L . Dynamic aspects of phospholipids during protein secretion. Int Rev Cytol. 1968; 23:187-208. DOI: 10.1016/s0074-7696(08)60272-7. View

11.

Porter R . The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959; 73:119-26. PMC: 1197021. DOI: 10.1042/bj0730119. View

12.

Resch K, FERBER E, Odenthal J, Fischer H . Early changes in the phospholipid metabolism of lymphocytes following stimulation with phytohemagglutinin and with lysolecithin. Eur J Immunol. 1971; 1(3):162-5. DOI: 10.1002/eji.1830010304. View

13.

Karnovsky M, Wallach D . The metabolic basis of phagocytosis. III. Incorporation of inorganic phosphate into various classes of phosphatides during phagocytosis. J Biol Chem. 1961; 236:1895-901. View

14.

Kai M, HAWTHORNE J . Incorporation of injected [32P] phosphate into the phosphoinositides of subcellular fractions from young rat brain. Biochem J. 1966; 98(1):62-7. PMC: 1264794. DOI: 10.1042/bj0980062. View

15.

Fisher D, Mueller G . An early alteration in the phospholipid metabolism of lymphocytes by phytohemagglutinin. Proc Natl Acad Sci U S A. 1968; 60(4):1396-402. PMC: 224932. DOI: 10.1073/pnas.60.4.1396. View

16.

Jones G . The number of reactive cells in mouse lymphocyte cultures stimulated by phytohemagglutinin, concanavalin A or histocompatibility antigen. J Immunol. 1973; 111(3):914-20. View

17.

Freinkel N, Dawson R . Role of inositol cyclic phosphate in stimulated tissues. Nature. 1973; 243(5409):535-7. DOI: 10.1038/243535a0. View

18.

Sharon N, Lis H . Lectins: cell-agglutinating and sugar-specific proteins. Science. 1972; 177(4053):949-59. DOI: 10.1126/science.177.4053.949. View

19.

Resch K, FERBER E . Phospholipid metabolism of stimulated lymphocytes. Effects of phytohemagglutinin, concanavalin A and anti-immunoglobulin serum. Eur J Biochem. 1972; 27(1):153-61. DOI: 10.1111/j.1432-1033.1972.tb01821.x. View

20.

Gordon J, Blumberg S, Lis H, Sharon N . Purification of soybean agglutinin by affinity chromatography On sepharose-N-epsilon-aminocaproyl-beta-D-galactopyranosylamine. FEBS Lett. 1972; 24(2):193-196. DOI: 10.1016/0014-5793(72)80765-8. View