Radiation Hybrid Mapping of Cataract Genes in the Dog

Overview

Journal Mol Vis

Specialties Cell Biology
Molecular Biology
Ophthalmology

Date 2006 Jun 9

PMID 16760895

Citations 4

Authors

Linda S Hunter

Duska J Sidjanin

Jennifer L Johnson

Barbara Zangerl

Francis Galibert

Catherine Andre

Ewen Kirkness

Elijah Talamas

Gregory M Acland

Gustavo D Aguirre

Affiliations

Soon will be listed here.

Abstract

Purpose: To facilitate the molecular characterization of naturally occurring cataracts in dogs by providing the radiation hybrid location of 21 cataract-associated genes along with their closely associated polymorphic markers. These can be used for segregation testing of the candidate genes in canine cataract pedigrees.

Methods: Twenty-one genes with known mutations causing hereditary cataracts in man and/or mouse were selected and mapped to canine chromosomes using a canine:hamster radiation hybrid RH5000 panel. Each cataract gene ortholog was mapped in relation to over 3,000 markers including microsatellites, ESTs, genes, and BAC clones. The resulting independently determined RH-map locations were compared with the corresponding gene locations from the draft sequence of the canine genome.

Results: Twenty-one cataract orthologs were mapped to canine chromosomes. The genetic locations and nearest polymorphic markers were determined for 20 of these orthologs. In addition, the resulting cataract gene locations, as determined experimentally by this study, were compared with those determined by the canine genome project. All genes mapped within or near chromosomal locations with previously established homology to the corresponding human gene locations based on canine:human chromosomal synteny.

Conclusions: The location of selected cataract gene orthologs in the dog, along with their nearest polymorphic markers, serves as a resource for association and linkage testing in canine pedigrees segregating inherited cataracts. The recent development of canine genomic resources make canine models a practical and valuable resource for the study of human hereditary cataracts. Canine models can serve as large animal models intermediate between mouse and man for both gene discovery and the development of novel cataract therapies.

Citing Articles

Looking the cow in the eye: deletion in the NID1 gene is associated with recessive inherited cataract in Romagnola cattle.

Murgiano L, Jagannathan V, Calderoni V, Joechler M, Gentile A, Drogemuller C PLoS One. 2014; 9(10):e110628.

PMID: 25347398 PMC: 4210201. DOI: 10.1371/journal.pone.0110628.

Association study of candidate genes for primary cataracts and fine-mapping of a candidate region on dog chromosome 1 in Entlebucher mountain dogs.

Muller C, Distl O Mol Vis. 2008; 14:883-8.

PMID: 18490961 PMC: 2386508.

Cloning and characterization of canine PAX6 and evaluation as a candidate gene in a canine model of aniridia.

Hunter L, Sidjanin D, Hijar M, Johnson J, Kirkness E, Acland G Mol Vis. 2007; 13:431-42.

PMID: 17417604 PMC: 2647561.

Evaluation of three canine gamma-crystallins (CRYGB, CRYGC, and CRYGS) as candidates for hereditary cataracts in the dachshund.

Muller C, Wohlke A, Distl O Mol Vis. 2007; 13:125-32.

PMID: 17327821 PMC: 2533037.

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