Cytosolic Inositol 1,4,5-trisphosphate Dynamics During Intracellular Calcium Oscillations in Living Cells

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2006 Jun 7

PMID 16754959

Citations 45

Authors

Toru Matsu-Ura

Takayuki Michikawa

Takafumi Inoue

Atsushi Miyawaki

Manabu Yoshida

Katsuhiko Mikoshiba

Affiliations

Soon will be listed here.

Abstract

We developed genetically encoded fluorescent inositol 1,4,5-trisphosphate (IP3) sensors that do not severely interfere with intracellular Ca2+ dynamics and used them to monitor the spatiotemporal dynamics of both cytosolic IP3 and Ca2+ in single HeLa cells after stimulation of exogenously expressed metabotropic glutamate receptor 5a or endogenous histamine receptors. IP3 started to increase at a relatively constant rate before the pacemaker Ca2+ rise, and the subsequent abrupt Ca2+ rise was not accompanied by any acceleration in the rate of increase in IP3. Cytosolic [IP3] did not return to its basal level during the intervals between Ca2+ spikes, and IP3 gradually accumulated in the cytosol with a little or no fluctuations during cytosolic Ca2+ oscillations. These results indicate that the Ca2+ -induced regenerative IP3 production is not a driving force of the upstroke of Ca2+ spikes and that the apparent IP3 sensitivity for Ca2+ spike generation progressively decreases during Ca2+ oscillations.

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