Endonuclease G: a Role for the Enzyme in Recombination and Cellular Proliferation

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2006 Jun 7

PMID 16754849

Citations 33

Authors

Ke-Jung Huang

Chia-Chi Ku

I Robert Lehman

Affiliations

Soon will be listed here.

Abstract

Our earlier studies had suggested that endonuclease G (EndoG), a member of the evolutionarily conserved DNA/RNA nonspecific betabetaalpha-Me-finger nuclease family, functioned in the a sequence-mediated segment inversion observed during herpes simplex virus 1 replication. To test this hypothesis, we used RNA interference to reduce the level of EndoG in mammalian cells in culture. Reduction of EndoG produced a small but statistically significant decrease in a sequence-mediated recombination, suggesting that EndoG does play a role in this process. We also observed that reduction in the level of EndoG resulted in a deficiency in cell proliferation. Cells with a reduced level of EndoG also showed changes in cell distribution in the cell cycle, producing a pattern characteristic of cells that have been arrested in the G(2) phase. These findings suggest that EndoG is required for normal cellular proliferation.

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