Suz12 Binds to Silenced Regions of the Genome in a Cell-type-specific Manner

Overview

Journal Genome Res

Specialty Genetics

Date 2006 Jun 6

PMID 16751344

Citations 166

Authors

Sharon L Squazzo

Henriette OGeen

Vitalina M Komashko

Sheryl R Krig

Victor X Jin

Sung-Wook Jang

Raphael Margueron

Danny Reinberg

Roland Green

Peggy J Farnham

Affiliations

Soon will be listed here.

Abstract

Suz12 is a component of the Polycomb group complexes 2, 3, and 4 (PRC 2/3/4). These complexes are critical for proper embryonic development, but very few target genes have been identified in either mouse or human cells. Using a variety of ChIP-chip approaches, we have identified a large set of Suz12 target genes in five different human and mouse cell lines. Interestingly, we found that Suz12 target promoters are cell type specific, with transcription factors and homeobox proteins predominating in embryonal cells and glycoproteins and immunoglobulin-related proteins predominating in adult tumors. We have also characterized the localization of other components of the PRC complex with Suz12 and investigated the overall relationship between Suz12 binding and markers of active versus inactive chromatin, using both promoter arrays and custom tiling arrays. Surprisingly, we find that the PRC complexes can be localized to discrete binding sites or spread through large regions of the mouse and human genomes. Finally, we have shown that some Suz12 target genes are bound by OCT4 in embryonal cells and suggest that OCT4 maintains stem cell self-renewal, in part, by recruiting PRC complexes to certain genes that promote differentiation.

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