Enzymic Hydrolysis of Sphingolipids: Hydrolysis of Ceramide Lactoside by an Enzyme from Rat Brain

Overview

Journal Biochem J

Specialty Biochemistry

Date 1966 Dec 1

PMID 16742444

Citations 6

Authors

S Gatt

M M Rapport

Affiliations

Soon will be listed here.

Abstract

Ceramide lactoside [1-O-(galactosido-4-beta-glucosido)-2-N-acyl-sphingosine] was hydrolysed to ceramide glucoside and galactose by beta-galactosidase of rat brain. The reaction was not reversible, required cholate or taurocholate, had optimum pH5.0 and K(m) 2.2x10(-5)m. It was inhibited by gamma-galactonolactone and galactose as well as by ceramide, sphingosine and fatty acid. Ceramide lactoside could be degraded to ceramide, galactose and glucose by mixtures of rat-brain beta-galactosidase and ox-brain beta-glucosidase.

Citing Articles

Ceramide function in the brain: when a slight tilt is enough.

Mencarelli C, Martinez-Martinez P Cell Mol Life Sci. 2012; 70(2):181-203.

PMID: 22729185 PMC: 3535405. DOI: 10.1007/s00018-012-1038-x.

Enzymic hydrolysis of sphingolipids: Hydrolysis of ceramide glucoside by an enzyme from ox brain.

Gatt S Biochem J. 1966; 101(3):687-91.

PMID: 16742445 PMC: 1270173.

Enzymic synthesis of cerebroside from ceramide and uridine diphosphate galactose.

Fujino Y, Nakano M Biochem J. 1969; 113(3):573-5.

PMID: 5807218 PMC: 1184705. DOI: 10.1042/bj1130573.

[Phospholipid metabolism and function of the mammalian brain in vivo. I. Catabolic changes of phospholipids in different parts of the rabbit brain during ischemia].

HINZEN D, Isselhard W, Fusgen I, Muller U Pflugers Arch. 1970; 318(2):119-29.

PMID: 5464604 DOI: 10.1007/BF00586491.

Hydrolysis of GM1-ganglioside by human liver beta-galactosidase isoenzymes.

Ho M, Cheetham P, Robinson D Biochem J. 1973; 136(2):351-9.

PMID: 4774399 PMC: 1165961. DOI: 10.1042/bj1360351.

References

Gatt S . Enzymic hydrolysis of sphingolipids: Hydrolysis of ceramide glucoside by an enzyme from ox brain. Biochem J. 1966; 101(3):687-91. PMC: 1270173. View

Gatt S, Rapport M . Isolation of beta-galactosidase and beta-glucosidase from brain. Biochim Biophys Acta. 1966; 113(3):567-76. DOI: 10.1016/s0926-6593(66)80014-0. View

Wiegandt H, BASCHANG G . [THE ISOLATION OF THE SUGAR PORTION OF GLYCOSPHINGOLIPIDS BY OZONOLYSIS AND FRAGMENTATION]. Z Naturforsch B. 1965; 20:164-6. View

Roth H, Segal S, Bertoli D . THE QUANTITATIVE DETERMINATION OF GALACTOSE--AN ENZYMIC METHOD USING GALACTOSE OXIDASE, WITH APPLICATIONS TO BLOOD AND OTHER BIOLOGICAL FLUIDS. Anal Biochem. 1965; 10:32-52. DOI: 10.1016/0003-2697(65)90238-1. View

Svennerholm L . THE GANGLIOSIDES. J Lipid Res. 1964; 5:145-55. View

SAMBASIVARAO K, McCluer R . THIN-LAYER CHROMATOGRAPHIC SEPARATION OF SPHINGOSINE AND RELATED BASES. J Lipid Res. 1963; 4:106-8. View

Klenk E, LIEDTKE U, Gielen W . [BRAIN GANGLIOSIDES IN INFANTILE AMAUROTIC IDIOCY OF THE TAY-SACHS TYPE]. Hoppe Seylers Z Physiol Chem. 1963; 334:186-92. DOI: 10.1515/bchm2.1963.334.1.186. View

SVENNERHOLM E, Svennerholm L . THE SEPARATION OF NEUTRAL BLOOD-SERUM GLYCOLIPIDS BY THIN-LAYER CHROMATOGRAPHY. Biochim Biophys Acta. 1963; 70:432-41. DOI: 10.1016/0006-3002(63)90773-x. View

Yamakawa T, Yokoyama S, KISO N . Structure of main globoside of human erythrocytes. J Biochem. 1962; 52:228-9. DOI: 10.1093/oxfordjournals.jbchem.a127603. View

10.

Svennerholm L . The chemical structure of normal human brain and Tay-Sachs gangliosides. Biochem Biophys Res Commun. 1962; 9:436-41. DOI: 10.1016/0006-291x(62)90030-x. View

11.

Gatt S, Berman E . Studies on brain lipids in Tay-Sachs' disease. III. Solubility properties of gangliosides. J Neurochem. 1963; 10:65-72. View

12.

Gatt S, Berman E . Studies on brain lipids in Tay-Sachs' disease. I. Isolation of two sialic acid-free glycolipids. J Neurochem. 1963; 10:43-9. DOI: 10.1111/j.1471-4159.1963.tb05036.x. View

13.

Rapport M, Schneider H, Graf L . Immunochemical studies of organ and tumor lipids. XI. A simplified preparation of cytolipin H. J Biol Chem. 1962; 237:1056-9. View

14.

Klenk E, DEBUCH H . The lipides. Annu Rev Biochem. 1959; 28:39-68. DOI: 10.1146/annurev.bi.28.070159.000351. View

15.

Rapport M, Graf L, ALONZO N . Immunochemical studies of organ and tumor lipids: VIII. Comparison of human tumor and ox spleen cytosides. J Lipid Res. 1960; 1:301-4. View

16.

Rapport M, Graf L, SKIPSKI V, ALONZO N . Immunochemical studies of organ and tumor lipids. VI. Isolation and properties of cytolipin H. Cancer. 1959; 12(3):438-45. DOI: 10.1002/1097-0142(195905/06)12:3<438::aid-cncr2820120303>3.0.co;2-o. View

17.

RADIN N . Glycolipide determination. Methods Biochem Anal. 1958; 6:163-89. DOI: 10.1002/9780470110225.ch7. View

18.

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

19.

Papavassiliou J . Hydrolysis of ceramide lactoside and ceramide glucoside by glycosidases from brain. Isr J Med Sci. 1965; 1(4):624-7. View

20.

HAJRA A, Bowen D, Kishimoto Y, RADIN N . Cerebroside galactosidase of brain. J Lipid Res. 1966; 7(3):379-86. View